LOCUS       Exported                4497 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Broad host range vector pBMTBX-5, complete sequence.
ACCESSION   GQ438222
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Broad host range vectors for expression in Gram negative hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2009) Chemical and Biological Engineering, 
            University of Colorado, UCB 424, Boulder, CO 80309, USA
REFERENCE   3  (bases 1 to 4497)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Broad host range vector pBMTBX-5"
                     /mol_type="other DNA"
                     /db_xref="taxon:671112"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR"
     terminator      60..96
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     CDS             complement(104..1123)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label=mob
                     /note="required for mobilization"
                     /protein_id="ACV83891.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(104..1123)
                     /gene="mob"
                     /label=mob
                     /note="mob gene from pBBR1MCS in INSD accession U02374"
     rep_origin      1347..2116
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2117..2779
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="required for replication"
                     /protein_id="ACV83892.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            2117..2779
                     /gene="rep"
                     /label=rep
                     /note="rep gene from pBBR1 in INSD accession X66730"
     CDS             2876..3112
                     /codon_start=1
                     /gene="dhfrIIb"
                     /product="dihydrofolate reductase"
                     /label=dhfrIIb
                     /protein_id="ACV83893.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     gene            2876..3112
                     /gene="dhfrIIb"
                     /label=dhfrIIb
                     /note="dhfrIIb gene from R388"
     regulatory      3129..3160
                     /regulatory_class="terminator"
                     /note="TonB"
     terminator      3129..3160
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(3188..4117)
                     /codon_start=1
                     /gene="araC"
                     /product="AraC"
                     /label=araC
                     /note="regulator"
                     /protein_id="ACV83894.1"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIEA
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPE
                     AREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGR
                     YSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVC
                     LSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRV
                     FKKCTGASPSEFRAGCEEKVNDVAVKLS"
     gene            complement(3188..4117)
                     /gene="araC"
                     /label=araC
     CDS             complement(3188..4066)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4093..4377
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      4125..4392
                     /regulatory_class="promoter"
                     /note="pBAD"