LOCUS       Exported                7949 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pBM6DraA6, complete sequence.
ACCESSION   LT727632
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7949)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Mammalian expression vector pBM6DraA6"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2207."
                     /db_xref="taxon:1944752"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..111)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     misc_feature    complement(170..1005)
                     /label=PY
                     /note="PY"
     rep_origin      complement(262..373)
                     /direction=LEFT
                     /label=PY-O
                     /note="PY-O"
     polyA_signal    1006..1140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1066..1162)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      1066..1162
                     /regulatory_class="terminator"
                     /note="polyA"
     misc_feature    complement(1163..1714)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1565..1585)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1715..1781)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1715..1780
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1782..1856)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL89035.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     CDS             complement(2201..2995)
                     /codon_start=1
                     /note="unnamed protein product; neomycin resistance"
                     /protein_id="SJL89036.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      complement(3354..3536)
                     /regulatory_class="promoter"
                     /note="LTR"
     promoter        complement(3355..3617)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     polyA_signal    3941..4075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(4001..4097)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      4001..4097
                     /regulatory_class="terminator"
                     /note="polyA"
     misc_feature    complement(4098..4649)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(4500..4520)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(4650..4716)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          4650..4715
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(4717..4791)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL89037.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     promoter        4799..4817
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      4799..4815
                     /regulatory_class="promoter"
                     /note="SP6"
     CDS             complement(5022..5657)
                     /codon_start=1
                     /note="unnamed protein product; mIL6"
                     /protein_id="SJL89038.1"
                     /translation="MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPV
                     YTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGY
                     NQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKI
                     VLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT"
     promoter        complement(5761..5779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(5763..5779)
                     /regulatory_class="promoter"
                     /note="T7"
     regulatory      complement(5825..6411)
                     /regulatory_class="promoter"
                     /note="hCMV"
     promoter        complement(5825..6028)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6029..6408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(6563..6765)
                     /label=supF
                     /note="supF"
     tRNA            complement(6567..6746)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     rep_origin      6889..7347
                     /label=M13-O
                     /note="M13-O"
     rep_origin      complement(7366..7598)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"