LOCUS       Exported                7143 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Insect expression vector pBlueBacmsGCA1His, complete sequence.
ACCESSION   LT727217
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7143)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Insect expression vector pBlueBacmsGCA1His"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4470."
                     /db_xref="taxon:1944738"
     promoter        4..95
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      7..95
                     /regulatory_class="promoter"
                     /note="AcNPV-PH promoter"
     CDS             215..2287
                     /codon_start=1
                     /note="unnamed protein product; msGCA1"
                     /protein_id="SJL87787.1"
                     /translation="MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLP
                     ICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEE
                     NRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFL
                     NSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAA
                     ARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASL
                     FCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMT
                     MLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLY
                     LSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIF
                     PSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQC
                     GELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIG
                     LHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFT
                     PRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGVD"
     misc_feature    2312..2353
                     /label=V5 epitope
                     /note="V5 epitope"
     CDS             2312..2353
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2363..2380
                     /label=His-tag
                     /note="His-tag"
     CDS             2363..2380
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2405..2526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2405..2479)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2405..2479
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     misc_recomb     2550..3255
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3UTR           2638..3010
                     /label=polyhedrin 3 UTR
                     /note="3 UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(2667..3545)
                     /codon_start=1
                     /note="unnamed protein product; 3 AcNPV ORF 1629"
                     /protein_id="SJL87788.1"
                     /translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPM
                     VDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTT
                     NLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTS
                     SQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPA
                     IGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQL
                     SVNQQRTESSA"
     CDS             complement(2667..3255)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     promoter        3828..3932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3933..4793
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87789.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4964..5552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5334..5728
                     /label=pMB1 ori
                     /note="pMB1 ori"
     CDS             complement(5729..6841)
                     /codon_start=1
                     /note="unnamed protein product; 5 lacZ fragment"
                     /protein_id="SJL87790.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6842..7116)
                     /regulatory_class="promoter"
                     /note="AcNPV-ETL promoter"