LOCUS       Exported                8219 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBlTAL, complete sequence.
ACCESSION   JF756587
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Anasontzis GE, Stathopoulou PM, Hatzinikolaou DG.
  TITLE     Homologous overexpression of transaldolase in Fusarium oxysporum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Anasontzis GE, Stathopoulou PM, Hatzinikolaou DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Laboratory of Microbiology, Sector of 
            Botany, Department of Biology, National and Kapodistrian University 
            of Athens, Zografou Campus, Zografou, Attiki 15784, Greece
REFERENCE   3  (bases 1 to 8219)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Expression vector pBlTAL"
                     /mol_type="other DNA"
                     /db_xref="taxon:1084984"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=f1(+) origin of ss-DNA replication
                     /note="f1(+) origin of ss-DNA replication"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      673..1031
                     /regulatory_class="promoter"
                     /note="trpC promoter from pCSN44"
     promoter        673..1029
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     gene            1040..2085
                     /gene="hygBr"
                     /label=hygBr
     CDS             1040..2065
                     /codon_start=1
                     /gene="hygBr"
                     /product="hygromycin resistance protein"
                     /label=hygBr
                     /protein_id="AEP25515.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     regulatory      2086..2803
                     /regulatory_class="terminator"
                     /note="trpC terminator from pCSN44"
     terminator      2235..2793
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     3086..3102
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3135..3738)
                     /regulatory_class="terminator"
                     /note="trpC terminator from Aspergillus nidulans"
     CDS             complement(join(3745..3978,4042..4425,4484..4767,4925..4936,
                     4996..5014,5237..5275))
                     /codon_start=1
                     /product="transaldolase"
                     /label=transaldolase
                     /note="from Fusarium oxysporum"
                     /protein_id="AEP71396.1"
                     /translation="MSSSLEQLKATGTTVVSDSGDFASIGKYKPQDATTNPSLILAASK
                     KAEYAKLIDVAIDYAKQKGGPIDQQVDDALDRLLVEFGKEILKIIPGKVSTEVDARYSF
                     DTEASVNKALHLIELYGEQGISKDRILIKIAATWEGIKAAEILQRDHGINTNLTLMFSL
                     VQAIGAAEAGAYLISPFVGRILDWFKASTKKEYSKEEDPGVQSVKTIFNYYKKYGYNTI
                     VMGASFRNTGEITELAGCDYLTISPNLLEELLNSNEPVPKKLDASQASSLDIEKKSYIN
                     DEALFRFDFNEDQMAVEKLREGISKFAADAVTLKSILKEKLA"
     regulatory      complement(5284..6004)
                     /regulatory_class="promoter"
                     /note="gpdA promoter from Aspergillus nidulans"
     promoter        complement(6031..6049)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6070..6086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6094..6110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6118..6148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6163..6184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      6416..7083
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     rep_origin      complement(6472..7060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7231..8091)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AEP25516.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8092..8196)
                     /gene="bla"
                     /label=AmpR promoter