LOCUS       Exported                4404 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pBLCAT5, complete sequence.
ACCESSION   LT727516
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Reporter vector pBLCAT5"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2451."
                     /db_xref="taxon:1945199"
     regulatory      39..143
                     /regulatory_class="promoter"
                     /note="HSV-TK promoter"
     promoter        112..174
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             242..901
                     /codon_start=1
                     /note="unnamed protein product; chloramphenicol resistance"
                     /protein_id="SJL88713.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             991..1065
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL88714.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    1066..1132
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1067..1132
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    1133..1684
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             1262..1282
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      complement(1685..1781)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      1685..1781
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    1707..1841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1877..1893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1901..1917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1920..2003)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(1925..1955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1970..1991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2095..2497)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2279..2867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3038..3898)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88715.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3899..4003)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4030..4164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(4030..4104)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      4030..4104
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      complement(4245..4341)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      4245..4341
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    4267..4401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"