low copy number in pir+ E. coli strains high copy number in pir116 E. coli strains
| 载体基本信息 | |||
| 载体名称 | pKNOCK-Km | 载体抗性 | Kanamycin |
|---|---|---|---|
| 载体长度 | 2098 bp | 载体类型 | Prokaryotic Expression Vectors |
pKNOCK-Km 载体载体图谱
pKNOCK-Km 载体载体序列
LOCUS Exported 2098 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2098)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..2098
/organism="synthetic DNA construct"
/mol_type="other DNA"
oriT 93..202
/note="incP origin of transfer"
rep_origin 373..761
/note="R6K gamma ori"
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
primer_bind 794..810
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(844..860)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
CDS 1258..2052
/codon_start=1
/gene="aph(3)-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
