LOCUS       Exported                3870 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBKHGC, complete sequence.
ACCESSION   KF378613
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Kang H-L., Jo S-U., Kwon S-U., Kwon S-U., Song J-Y., Seo J-H., Cho 
            M-J., Baik S-C., Youn H-S., Rhee K-H., Lee W-K.
  TITLE     A new expression vector for the rapid purification of recombinant 
            proteins from Helicobacter pylori
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Kang H-L., Jo S-U., Kwon S-U., Kwon S-U., Song J-Y., Seo J-H., Cho 
            M-J., Baik S-C., Youn H-S., Rhee K-H., Lee W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Department of Microbiology, Gyeongsang 
            National University School of Medicine, Jinju, Gyeongnam 660-751, 
            Korea
REFERENCE   3  (bases 1 to 3870)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: DNASTAR v. ver1.0
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Expression vector pBKHGC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1414449"
     primer_bind     1..17
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    28..45
                     /label=thrombin recognition site
                     /note="thrombin recognition site"
     CDS             28..45
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    46..75
                     /label=6 histidine tag
                     /note="6 histidine tag"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..756
                     /codon_start=1
                     /product="glutathione S-transferase"
                     /label=glutathione S-transferase
                     /note="gst"
                     /protein_id="AGZ15598.1"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYMADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLV
                     PR"
     CDS             82..735
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYMADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             763..1386
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="cat"
                     /protein_id="AGZ15599.1"
                     /translation="MQFTKIDINNWTRKEYFDHYFGNTPCTYSMTVKLDISKLKKDGKK
                     LYPTLLYGVTTIINRHEEFRTALDENGQVGVFSEMLPCYTVFHKETETFSSIWTEFTAD
                     YTEFLQNYQKDIDAFGERMGMSAKPNPPENTFPVSMIPWTSFEGFNLNLKKGYDYLLPI
                     FTFGKYYEEGGKYYIPLSIQVHHAVCDGFHVCRFLDELQDLLNK"
     CDS             complement(1417..2211)
                     /codon_start=1
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=aminoglycoside 3-phosphotransferase
                     /protein_id="AGZ15600.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"