LOCUS       Exported                7441 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBK638, complete sequence.
ACCESSION   DQ333233
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Schoep TD, Gregg K.
  TITLE     Primer Extension Analysis of Gene Promoters by Fluorescent DNA 
            Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Schoep TD, Gregg K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2005) Microbiology and Immunology, University of 
            Western Australia, Stirling Hwy, Perth, WA 6009, Australia
REFERENCE   3  (bases 1 to 7441)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Shuttle vector pBK638"
                     /lab_host="Butyrivibrio fibrisolvens and Escherichia coli"
                     /mol_type="other DNA"
                     /label=promoter rescue plasmid
                     /note="promoter rescue plasmid"
                     /db_xref="taxon:362869"
     regulatory      19..338
                     /regulatory_class="terminator"
                     /note="bacteriophage T4D gene 32 transcription/translation 
                     terminator"
     regulatory      339..875
                     /regulatory_class="promoter"
     regulatory      568..573
                     /regulatory_class="minus_35_signal"
     regulatory      591..596
                     /regulatory_class="minus_10_signal"
     regulatory      680..685
                     /regulatory_class="minus_35_signal"
     regulatory      703..708
                     /regulatory_class="minus_10_signal"
     CDS             897..1520
                     /codon_start=1
                     /gene="ermAM"
                     /product="erythromycin resistance protein"
                     /label=ermAM
                     /protein_id="ABD13962.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRCTLDIHRTLG
                     LLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNRE
                     YRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            897..1520
                     /gene="ermAM"
                     /label=ermAM
     CDS             2052..2870
                     /codon_start=1
                     /gene="kanR"
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=kanR
                     /note="kanamycin resistance"
                     /protein_id="ABD13963.1"
                     /translation="MSHIQRETSCSTRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            2052..2870
                     /gene="kanR"
                     /label=kanR
     rep_origin      3013..3601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3889..3910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    3965..3981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3989..4005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4043..4061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4068..4084)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4792..4808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5278..6132)
                     /codon_start=1
                     /product="ORF2"
                     /label=ORF2
                     /protein_id="ABD13964.1"
                     /translation="MSHNMILLFSEGITMKNDDRMEIAIERDYKVVKANEIIQKAKFDL
                     GLLEQKTFCYAVSKIKPDDALGSEYVFTINEYCDICGINRNSGKTIEEVKSALKRLRDK
                     SFYMLDEKGDYVLIGWLSKAKVSPRSGKIAIKFDEDMQKYLMGLYTNYTQYSLLCVLPM
                     RSTYSIRLYELLKSYAGLHKKDFDIDDLKAQLCAPYARFPDFRRKVIEVAVKEINIYTD
                     IEVSWEPINKGRKVVKVHFSIKQRNSMKIAMAGARATEALDGIDGQIRFDLEGNLSEER
                     KSR"
     CDS             complement(6393..6830)
                     /codon_start=1
                     /product="putative replication protein"
                     /label=putative replication protein
                     /note="ORF1"
                     /protein_id="ABD13965.1"
                     /translation="MTITEFAESRQVQPQAISRYIGRHPEKFNGHTEKKGKTVELDDIA
                     LELLEKKYPMPAPVQIIEDTESRQKLIKAQELIIQLQDKLMDAQSQIAEAEATKILLED
                     KNAQIEKYELTEANYKKQIDELLEELSKEKSKTWIDKLFKK"