LOCUS       Exported               13144 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Transformation vector pBISN1-IN, complete sequence.
ACCESSION   EU886197
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Abdeeva IA, Abdeev RM, Piruzian ES.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2008) Laboratory of Functional Genomics, Vavilovs
            Institute of General Genetics Russian Academy of Science, Gubkina, 
            Moscow 119991, Russia
REFERENCE   2  (bases 1 to 13144)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Transformation vector pBISN1-IN"
                     /mol_type="other DNA"
                     /note="Escherichia coli/Agrobacterium tumefaciens 
                     transformation vector"
                     /db_xref="taxon:553236"
     mobile_element  1317..2084
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     CDS             2276..3070
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3369..4517
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(6043..6190)
                     /label=T-DNA level border
                     /note="T-DNA level border"
     misc_feature    6113..6137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6755..6771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(6773..6977)
                     /regulatory_class="polyA_signal_sequence"
     CDS             complement(7000..7659)
                     /codon_start=1
                     /gene="licBM2"
                     /product="lichenase"
                     /label=licBM2
                     /note="beta-1,3-1,4-glucanase"
                     /protein_id="ACI16381.1"
                     /translation="MVNTPFVAVFSNFDSSQWEKADWANGSVFNCVWKPSQVTFSNGKM
                     ILTLDREYGGSYPYKSGEYRTKSFFGYGYYEVRMKAAKNVGIVSSFFTYTGPSDNNPWD
                     EIDIEFLGKDTTKVQFNWYKNGVGGNEYLHNLGFDASQDFHTYGFEWRPDYIDFYVDGK
                     KVYRGTRNIPVTPGKIMMNLWPGIGVDEWLGRYDGRTPLQAEYEYVKYYPNGKDEL"
     gene            complement(7000..7659)
                     /gene="licBM2"
                     /label=licBM2
     sig_peptide     complement(7003..7014)
                     /gene="licBM2"
                     /note="signal peptide KDEL tailed"
     misc_feature    complement(7662..7732)
                     /label=leader signal of the Vicia faba leB4
                     /note="leader signal of the Vicia faba leB4"
     regulatory      complement(7733..8181)
                     /regulatory_class="promoter"
                     /note="35S CaMV promoter"
     promoter        complement(7739..7786)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             7768..7779
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8209..8225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8233..8249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8257..8287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8302..8323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      complement(8868..9123)
                     /regulatory_class="terminator"
                     /note="NOS termination signal"
     terminator      8871..9123
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9513..10307)
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /label=nptII
                     /protein_id="ACI16380.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(9513..10307)
                     /gene="nptII"
                     /label=nptII
     regulatory      complement(10320..10626)
                     /regulatory_class="promoter"
                     /note="Pnos promoter"
     promoter        complement(10328..10511)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(10364..10543)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(10627..10788)
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    10667..10691
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11156..11806
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     oriT            12487..12596
                     /note="incP origin of transfer"
     CDS             12629..13000
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      13127..694
                     /label=oriV
                     /note="incP origin of replication"