LOCUS       Exported               12703 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pBIB-KAN, complete sequence.
ACCESSION   GU982971
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Condori J, Medina-Bolivar F.
  TITLE     Vector for transient expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   Condori J, Medina-Bolivar F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2010) Arkansas Biosciences Institute, Arkansas 
            State University, 504 University Loop East, Jonesboro, AK 72401, USA
REFERENCE   3  (bases 1 to 12703)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Binary vector pBIB-KAN"
                     /mol_type="other DNA"
                     /db_xref="taxon:744262"
     rep_origin      1..618
                     /note="oriV; similar to GenBank Accession Number M20134"
     misc_feature    1316..2085
                     /label=IS1: similar to GenBank Accession Number X58999
                     /note="IS1: similar to GenBank Accession Number X58999"
     mobile_element  1317..2084
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     CDS             2276..3070
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3369..4517
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     repeat_region   6113..6137
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    6113..6137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6755..6771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     3UTR           6780..7032
                     /label=derived from pAnos gene
                     /note="derived from pAnos gene"
     terminator      6780..7032
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      7405..7692
                     /regulatory_class="promoter"
                     /note="derived from Pnos gene"
     promoter        7488..7667
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        7520..7703
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7724..8518
                     /codon_start=1
                     /gene="nptII"
                     /product="nptII"
                     /label=nptII
                     /note="selectable marker for plant"
                     /protein_id="ADE08583.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            7724..8518
                     /gene="nptII"
                     /label=nptII
     regulatory      8908..9162
                     /regulatory_class="terminator"
                     /note="derived from Tnos gene"
     terminator      8908..9160
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9709..9730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9745..9775
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9783..9799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9807..9823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     3UTR           9859..10065
                     /label=derived from pAg7 gene
                     /note="derived from pAg7 gene"
     terminator      9865..10065
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     repeat_region   10226..10250
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    10226..10250
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10715..11365
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      11536..11914
                     /label=derived from ColE1 gene
                     /note="derived from ColE1 gene"
     oriT            12046..12155
                     /note="incP origin of transfer"
     CDS             12188..12559
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      12686..694
                     /label=oriV
                     /note="incP origin of replication"