LOCUS       Exported               11619 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pBIB-HYG, complete sequence.
ACCESSION   JX625145
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Condori JM, Medina-Bolivar F.
  TITLE     Vector for transient expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Condori JM, Medina-Bolivar F.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2012) Molecular Cloning, GeneCoMe Biotech LC, P.O.
            Box 19538, Jonesboro, AR 72403, USA
REFERENCE   3  (bases 1 to 11619)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Binary vector pBIB-HYG"
                     /mol_type="other DNA"
                     /db_xref="taxon:1247112"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     rep_origin      complement(790..1168)
                     /direction=LEFT
                     /note="similar to sequence in INSD accession V00268; ColE1"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     repeat_region   2454..2478
                     /rpt_family="T-DNA border"
                     /rpt_unit_range=2454 .. 2478
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      complement(2632..2857)
                     /regulatory_class="terminator"
                     /note="derived from gene 7 of the Agrobacterium tumefaciens
                     octopine ti-plasmid (Gielen et al. 1984, EMBO J. 3:835-846)
                     of INSD accessions V00090 and X00493; g7 polyA signal 
                     sequence"
     3UTR           2639..2845
                     /gene="pAg7"
                     /label=g7 (gene 7) poly A signal sequence
                     /note="g7 (gene 7) poly A signal sequence"
     gene            2639..2845
                     /gene="pAg7"
                     /label=pAg7
     terminator      2639..2839
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(2874..3899)
                     /codon_start=1
                     /gene="HPT"
                     /product="aminocyclitol phosphotransferase"
                     /function="hygromycin resistance"
                     /label=HPT
                     /protein_id="AFV57334.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            complement(2874..3899)
                     /gene="HPT"
                     /label=HPT
     regulatory      complement(3928..4215)
                     /regulatory_class="promoter"
                     /gene="Pnos"
                     /note="derived from the noplaine synthase gene (Depicker et
                     al. 1982, J.Mol.Appl.Genet. 1:561-573); INSD accession 
                     V00087"
     gene            complement(3928..4215)
                     /gene="Pnos"
                     /label=Pnos
     promoter        complement(3953..4132)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     3UTR           4588..4840
                     /gene="pAnos"
                     /label=3UTR of the nopaline synthase gene
                     /note="3UTR of the nopaline synthase gene"
     gene            4588..4840
                     /gene="pAnos"
                     /label=pAnos
     terminator      4588..4840
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4849..4865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5483..5507
                     /label=left T-DNA border
                     /rpt_unit_range=5483 .. 5507
                     /note="left T-DNA border"
     misc_feature    5483..5507
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(7103..8251)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(8542..10655)
                     /note="nptII cassette; selectable marker"
     CDS             complement(8550..9344)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    complement(9535..10304)
                     /note="similar to transposable element IS1 in INSD 
                     accession X58999; IS1"
     mobile_element  9536..10303
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     rep_origin      10926..18
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      complement(11002..11619)
                     /direction=LEFT
                     /note="similar to sequence in INSD accession M20134; oriV"