LOCUS       Exported                5038 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBhSV-2, complete sequence.
ACCESSION   EF488742
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Shuttle vector pBhSV-2"
                     /mol_type="other DNA"
                     /note="Borrelia hermsii/Escherichia coli shuttle vector 
                     conferring kanamycin resistance"
                     /db_xref="taxon:456494"
     rep_origin      1..719
                     /label=colE1 origin of replication
                     /note="colE1 origin of replication"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(787..1161)
                     /codon_start=1
                     /gene="zeoR"
                     /product="zeocin resistance protein"
                     /label=zeoR
                     /protein_id="ABS71228.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            complement(787..1161)
                     /gene="zeoR"
                     /label=zeoR
     regulatory      1158..1167
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(1418..2227)
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin resistance protein"
                     /label=kanR
                     /protein_id="ABS71229.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1418..2227)
                     /gene="kanR"
                     /label=kanR
     regulatory      complement(2228..2348)
                     /regulatory_class="promoter"
                     /note="flgB promoter derived from Borrelia hermsii"
     gene            complement(2605..3114)
                     /pseudo
                     /gene="vmp"
                     /label=vmp
                     /note="orfC"
     RBS             2684..2692
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     CDS             3281..4375
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="similar to gene family 57; orfA"
                     /protein_id="ABS71230.1"
                     /translation="MGNTKKFTNKYQHKLIVLISTLNYMNLKLKKYTQNDILYYFNNNM
                     KKNDQNPIKLKTLQSYLYKLKKEFQVTINYHRHLGVNMGTEIHYELKYSKKECYCIINK
                     QFREKKEERHKKRVNVYLEKTCIKNSSVEKWECSYNIYNNKEEKKDIKEIEKLQVKKYI
                     RKCNFKSDILYSILDLELEKNATIKVCKIIKRTENFIENSIYKRINGIKSNRSKQRELS
                     KILNETRIRLENEGHNGKQLETQIQEVYEQYKNKPHFIIENNKYNDLKKIIGKLKKTVE
                     YTNRNAKENERDVRNNVFSILLEQLRHKVDKSILVSILKGYLNKQDKLTYSKALNNYYY
                     HELLELINNNLDYLKPEKLEIITS"
     CDS             4385..4945
                     /codon_start=1
                     /gene="orfB"
                     /product="hypothetical protein"
                     /label=orfB
                     /note="simlar to gene family 50; orfB"
                     /protein_id="ABS71231.1"
                     /translation="MESILERLKKKESEIKKKNNRNLFVKVEKINNRTIYHTKIMKDLF
                     SFGINKNQRGKFFVSFRELFNQEKIAVFNLFSLRDDDKFLGISYWYRKPIQNVVTRYEE
                     NGIMKASTFSKVYYVEFRFKKGSVCCYIGEITYLLRKEKANKKYYESLVERMINLEKQV
                     YEFYGKKLPNGGIINKWIEKNQK"
     gene            4385..4945
                     /gene="orfB"
                     /label=orfB