LOCUS       Exported                3337 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBHK18, complete sequence.
ACCESSION   AY219691
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3337)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Shuttle vector pBHK18"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:224623"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             584..1378
                     /codon_start=1
                     /gene="aph(3)-IIa"
                     /product="Aph(3)-IIa"
                     /label=aph(3)-IIa
                     /note="aminoglycoside phosphotransferase"
                     /protein_id="AAO65216.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            584..1378
                     /gene="aph(3)-IIa"
                     /label=aph(3)-IIa
     CDS             1601..3052
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="AAO65217.1"
                     /translation="MSLQTHAQNAHTKTPTRVERDRGGLDNTPASASDRDLLRDHLGRD
                     VLHGSVTRDFKKAYRRNADGTNSPRMYRFETDALGRCEYAMLTTKQYAAVLVVDVDQVG
                     TAGGDPADLNPYVRDVVRSLITHSVGPAWVGINPTNGKAQFIWLIDPVYADRNGKSAQM
                     KLLAATTRVLGELLDHDPHFSHRFSRNPFYTGKAPTAYRWYRQHNRVMRLGDLIKQVRD
                     MAGHDQFNPTPRQQFSSGRELINAVKTRREEAQAFKALAQDVDAEIAGGLDQYDPELID
                     GVRVLWIVQGTAARDETAFRHALKTGHRLRQQGQRLTDAAIIDAYEHAYNVAHTHGGAG
                     RDNEMPPMRDRQTMARRVRGYVAQSKSETYSGSNAPGKATSSERKALATMGRRGGQKAA
                     QRWKTDPEGKYAQAQRSKLEKTHRKKKAQGRSTKSRISQMVNDQYFQTGTVPTWAEIGA
                     EVGVSRATVARHVAELKKSGDYPDV"
     gene            1601..3052
                     /gene="repA"
                     /label=repA
     promoter        3251..3281
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3289..3305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3313..3329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"