LOCUS       Exported               11160 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGT1, complete sequence.
ACCESSION   EU594544
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Lukacsovich T, Hamada N, Miyazaki S, Kimpara A, Yamamoto D.
  TITLE     A new versatile gene-trap vector for insect transgenics
  JOURNAL   Arch. Insect Biochem. Physiol. 69 (4), 168-175 (2008)
  PUBMED    18949801
REFERENCE   2  (bases 1 to 11160)
  AUTHORS   Lukacsovich T, Hamada N, Yamamoto D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2008) Developmental and Cell Biology, University 
            of California Irvine, Rm 4444, McGaugh Hall, Irvine, CA 92697-2300, 
            USA
REFERENCE   3  (bases 1 to 11160)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Cloning vector pBGT1"
                     /mol_type="other DNA"
                     /db_xref="taxon:519059"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_structure  1098..2149
                     /label=PiggyBac right end
                     /note="PiggyBac right end"
     misc_feature    2169..2187
                     /label=splice acceptor site
                     /note="splice acceptor site"
     CDS             2207..4852
                     /codon_start=1
                     /gene="Gal4"
                     /product="Gal4"
                     /label=Gal4
                     /note="transcription factor"
                     /protein_id="ACC38293.1"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     gene            2207..4852
                     /gene="Gal4"
                     /label=Gal4
     regulatory      5106..5348
                     /regulatory_class="terminator"
                     /note="hsp70T"
     regulatory      complement(5384..5619)
                     /regulatory_class="terminator"
                     /note="SV40T"
     polyA_signal    5385..5506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5627..6307)
                     /codon_start=1
                     /gene="dsRed"
                     /product="red fluorescent protein"
                     /label=dsRed
                     /protein_id="ACC38294.1"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     gene            complement(5627..6307)
                     /gene="dsRed"
                     /label=dsRed
     regulatory      6304..6313
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      complement(6360..6565)
                     /regulatory_class="promoter"
                     /note="3xP3-hsp70"
     regulatory      6618..7335
                     /regulatory_class="promoter"
                     /note="ie+hr"
     regulatory      7344..7353
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7350..8069
                     /codon_start=1
                     /gene="EGFP"
                     /product="green fluorescent protein"
                     /label=EGFP
                     /protein_id="ACC38295.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            7350..8069
                     /gene="EGFP"
                     /label=EGFP
     misc_feature    8069..8077
                     /label=splice donor site
                     /note="splice donor site"
     misc_structure  8079..8757
                     /label=PiggyBac left end
                     /note="PiggyBac left end"
     primer_bind     complement(8939..8955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8987..9017)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9032..9053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9341..9929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(9342..9823)
                     /direction=LEFT
                     /label=E. coli replication origin
                     /note="E. coli replication origin"
     CDS             complement(10100..10960)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin antibiotic resistance"
                     /protein_id="ACC38296.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10961..11065)
                     /gene="bla"
                     /label=AmpR promoter