LOCUS       Exported                7882 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGEN02, complete sequence.
ACCESSION   JQ280534
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Cloning vectors for achieving high levels of transgene expression in
            plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2011) Genetics, University of Delhi, South Campus,
            Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE   3  (bases 1 to 7882)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Cloning vector pBGEN02"
                     /mol_type="other DNA"
                     /db_xref="taxon:1165863"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4063
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5814..5838
                     /label=left T-DNA border
                     /note="left T-DNA border"
     misc_feature    6152..6185
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(6152..6185)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6199..6236
                     /note="multiple cloning site 1 (between loxP for cloning of
                     marker gene)"
     misc_feature    6252..6285
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(6252..6285)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6539..6805
                     /label=multiple cloning site 3 (outside loxP)
                     /note="multiple cloning site 3 (outside loxP)"
     promoter        6877..7223
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      6877..7221
                     /regulatory_class="promoter"
                     /note="35S"
     misc_feature    7253..7274
                     /note="multiple cloning site 2 (between 35S promoter and 
                     polyA for cloning of transgene)"
     regulatory      7276..7478
                     /regulatory_class="polyA_signal_sequence"
                     /note="35S"
     misc_feature    7476..7861
                     /note="multiple cloning site 4 (for cloning another 
                     transgene)"
     misc_feature    7746..7771
                     /label=right T-DNA border
                     /note="right T-DNA border"
     misc_feature    7746..7770
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"