LOCUS       Exported                9621 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGEN02-hpt, complete sequence.
ACCESSION   JQ280535
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Cloning vectors for achieving high levels of transgene expression in
            plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2011) Genetics, University of Delhi, South Campus,
            Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE   3  (bases 1 to 9621)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Cloning vector pBGEN02-hpt"
                     /mol_type="other DNA"
                     /db_xref="taxon:1165865"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4063
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5814..5838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    5814..5837
                     /label=left T-DNA border
                     /note="left T-DNA border"
     misc_feature    6152..6185
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(6152..6185)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6231..6236
                     /label=multiple cloning site 2 (between loxP)
                     /note="multiple cloning site 2 (between loxP)"
     regulatory      6297..6641
                     /regulatory_class="promoter"
                     /note="35S"
     promoter        6297..6641
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6669..7694
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /label=hygromycin phosphotransferase
                     /protein_id="AFF18868.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     regulatory      7710..7929
                     /regulatory_class="polyA_signal_sequence"
                     /note="35S"
     misc_feature    7991..8024
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(7991..8024)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    8278..8543
                     /label=multiple cloning site 3 (outside loxP)
                     /note="multiple cloning site 3 (outside loxP)"
     regulatory      8616..8962
                     /regulatory_class="promoter"
                     /note="35S"
     promoter        8616..8962
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    8986..8999
                     /note="multiple cloning site 1 (between 35S promoter and 
                     polyA)"
     misc_feature    9215..9318
                     /note="multiple cloning site 4 (for cloning another 
                     transgene)"
     misc_feature    9485..9510
                     /label=right T-DNA border
                     /note="right T-DNA border"
     misc_feature    9485..9509
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"