LOCUS       Exported               10549 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGEN02-ALSdm, complete sequence.
ACCESSION   JQ280536
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Cloning vectors for achieving high levels of transgene expression in
            plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2011) Genetics, University of Delhi, South Campus,
            Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE   3  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Cloning vector pBGEN02-ALSdm"
                     /mol_type="other DNA"
                     /db_xref="taxon:1165864"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     regulatory      2452..2667
                     /regulatory_class="polyA_signal_sequence"
                     /note="35S"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4063
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5814..5838
                     /label=left T-DNA border
                     /note="left T-DNA border"
     misc_feature    6152..6185
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(6152..6185)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      6219..6641
                     /regulatory_class="promoter"
                     /note="35S"
     promoter        6595..6641
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      6651..6660
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      6655..6664
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6657..8669
                     /codon_start=1
                     /gene="ALSdm"
                     /product="ALSdm"
                     /label=ALSdm
                     /protein_id="AFF18869.1"
                     /translation="MAAATTTTTTSSSISFSTKPSPSSSKSPLPISRFSLPFSLNPNKS
                     SSSSRRRGIKSSSPSSISAVLNTTTNVTTTPSPTKPTKPETFISRFAPDQPRKGADILV
                     EALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPGI
                     CIATSGPGATNLVSGLADALLDSVPLVAITGQVSRRMIGTDAFQETPIVEVTRSITKHN
                     YLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMP
                     KPPEDSHLEQIVRLISESKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGLGSYPC
                     DDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSA
                     EIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNELNVQKQKFPLSFKTF
                     GEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGL
                     PAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQWED
                     RFYKANRAHTFLGDPAQEDEIFPNMLLFAAACGIPAARVTKKADLREAIQTMLDTPGPY
                     LLDVICPHQEHVLPMIPNGGTFNDVITEGDGRIKY"
     gene            6657..8669
                     /gene="ALSdm"
                     /label=ALSdm
     regulatory      8686..8902
                     /regulatory_class="polyA_signal_sequence"
                     /note="35S"
     misc_feature    8919..8952
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(8919..8952)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    9206..9471
                     /label=multiple cloning site 2 (outside loxP)
                     /note="multiple cloning site 2 (outside loxP)"
     promoter        9544..9890
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      9544..9888
                     /regulatory_class="promoter"
                     /note="35S"
     misc_feature    9921..9941
                     /note="multiple cloning site 1 (between 35S promoter and 
                     polyA)"
     misc_feature    10143..10240
                     /note="multiple cloning site 3 (for cloning another 
                     transgene)"
     misc_feature    10413..10438
                     /label=right T-DNA border
                     /note="right T-DNA border"
     misc_feature    10413..10437
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"