LOCUS       Exported                6534 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBEVY-U, complete sequence.
ACCESSION   AF069724
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Miller CA III, Martinat MA, Hyman LE.
  TITLE     Assessment of Aryl Hydrocarbon Receptor Complex Interactions Using 
            pBEVY Plasmids: Expression Vectors With Bi-directional Promoters For
            Saccharomyces cerevisiae
  JOURNAL   Nucleic Acids Res. (1998) In press
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Miller CA III.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-1998) Environmental Health Sciences, Tulane 
            University School of Public Health and Tropical Medicine, 1430 
            Tulane Ave., New Orleans, LA 70112, USA
REFERENCE   3  (bases 1 to 6534)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Expression vector pBEVY-U"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:77616"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      233..585
                     /regulatory_class="terminator"
                     /note="ADH1 terminator"
     terminator      267..454
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     regulatory      complement(616..1279)
                     /regulatory_class="promoter"
                     /note="GPD promoter"
     promoter        complement(623..1268)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     regulatory      1280..1680
                     /regulatory_class="promoter"
                     /note="ADH1 promoter"
     regulatory      1691..2032
                     /regulatory_class="terminator"
                     /note="ADH2 terminator"
     terminator      1712..2011
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      2036..3383
                     /label=2-micron plasmid origin
                     /note="2-micron plasmid origin"
     rep_origin      2036..3382
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3493..4296)
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine monophosphate decarboxylase"
                     /label=URA3
                     /note="Saccharomyces cerevisiae Ura3p"
                     /protein_id="AAC23882.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            complement(3493..4296)
                     /gene="URA3"
                     /label=URA3
     promoter        complement(4297..4512)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        4628..4732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4733..5593
                     /codon_start=1
                     /gene="Bla"
                     /product="beta-lactamase"
                     /label=Bla
                     /note="ampicillin resistance protein"
                     /protein_id="AAC23881.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4733..5593
                     /gene="Bla"
                     /label=Bla
     rep_origin      5764..6352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      6165..6526
                     /label=ColE1 plasmid origin
                     /note="ColE1 plasmid origin"