LOCUS       Exported                6649 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBEVY-GL, complete sequence.
ACCESSION   AF069719
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Miller CA III, Martinat MA, Hyman LE.
  TITLE     Assessment of Aryl Hydrocarbon Receptor Complex Interactions Using 
            pBEVY Plasmids: Expression Vectors With Bi-directional Promoters For
            Saccharomyces cerevisiae
  JOURNAL   Nucleic Acids Res. (1998) In press
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Miller CA III.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-1998) Environmental Health Sciences, Tulane 
            University School of Public Health and Tropical Medicine, 1430 
            Tulane Ave., New Orleans, LA 70112, USA
REFERENCE   3  (bases 1 to 6649)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Expression vector pBEVY-GL"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:77611"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      233..585
                     /regulatory_class="terminator"
                     /note="ADH1 terminator"
     terminator      267..454
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     regulatory      616..1293
                     /regulatory_class="promoter"
                     /note="GAL1,10 bi-directional promoter"
     promoter        624..1288
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    840..957
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     regulatory      1304..1645
                     /regulatory_class="terminator"
                     /note="ADH2 terminator"
     terminator      1325..1624
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      1649..2996
                     /label=2-micron plasmid origin
                     /note="2-micron plasmid origin"
     rep_origin      1649..2995
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3133..4227)
                     /codon_start=1
                     /gene="LEU2"
                     /product="isopropylmalate dehydrogenase"
                     /label=LEU2
                     /note="Saccharomyces cerevisiae Leu2p"
                     /protein_id="AAC23871.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     gene            complement(3133..4227)
                     /gene="LEU2"
                     /label=LEU2
     promoter        4743..4847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4848..5708
                     /codon_start=1
                     /gene="Bla"
                     /product="beta-lactamase"
                     /label=Bla
                     /note="ampicillin resistance protein"
                     /protein_id="AAC23872.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4848..5708
                     /gene="Bla"
                     /label=Bla
     rep_origin      5879..6467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      6280..6641
                     /label=ColE1 plasmid origin
                     /note="ColE1 plasmid origin"