LOCUS       Exported                4791 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBEM3, complete sequence.
ACCESSION   HM352833
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Park SY, Kim GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Biology, Chonnam National University, 
            Yongbong-Dong 300, Buk-Gu, Gwangju, Chonnam 500-757, Korea
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Cloning vector pBEM3"
                     /mol_type="other DNA"
                     /db_xref="taxon:887794"
     misc_feature    4..2405
                     /label=BMS whole expression module
                     /note="BMS whole expression module"
     regulatory      2022..2071
                     /regulatory_class="promoter"
                     /note="BMS promoter"
     terminator      2619..2705
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2797..2824
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2844..2935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2936..3796
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ADN52080.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"