LOCUS       Exported                8202 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBCGL1, complete sequence.
ACCESSION   AY949861
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Govan VA, Christensen ND, Berkower C, Jacobs WR Jr., Williamson A-L.
  TITLE     Recombinant BCG expressing cottontail rabbit papillomavirus (CRPV) 
            L1 gene provides partial protection in rabbits challenged against 
            CRPV
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Govan VA, Christensen ND, Berkower C, Jacobs WR Jr., Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2005) Division of Medical Virology, Institute of 
            Infectious Diseases and Molecular Medicine, Anzio Road, Observatory,
            Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 8202)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Expression vector pBCGL1"
                     /mol_type="other DNA"
                     /db_xref="taxon:317473"
     CDS             1877..2692
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6556..8073
                     /codon_start=1
                     /gene="L1"
                     /product="CRPV L1 major capsid protein"
                     /label=L1
                     /note="derived from cottontail rabbit papillomavirus"
                     /protein_id="AAX37308.1"
                     /translation="MAVWLSTQNKFYLPPQPVTKIPSTDEYVTRTNVFYYASSDRLLTV
                     GHPYYEIRDKGTMLVPKVSPNQYRVFRIKLPDPNKFAFGDKQLYDPEKERLVWCLRGIE
                     VNRGQPLGVSVTGNPIFNKFDDVENPTKYYNNHADQQDYRKSMAFDPKQVQLLMLGCVP
                     ATGEHWAQAKQCAEDPPQQTDCPPIELVNTVIEDGDMCEIGFGAMDHKTLQASLSEVPL
                     ELAQSISKYPDYLKMQKDQFGDSMFFYARREQMYARHFFSRAGGDKENVKSRAYIKRTQ
                     MQGEANANIATDNYCITPSGSLVSSDSQVFNRAYWLQKAQGMNNGVCWDNQIFVTVVDN
                     TRGTILSLVTKSKEQIKKTHGKTVHFSSYLRHVEEYELQFVLQLCKVKLTPENLSYLHS
                     MHPTIIDNWQLSVSAQPSGTLEDQYRYLQSIATKCPPPEPPKENTDPYKNYKFWEVDLS
                     EKLSDQLDQYPLGRKFLNQSGLQRIGTKRPAPAPVSIVKSSKRKRRT"
     gene            6556..8073
                     /gene="L1"
                     /label=L1
     terminator      8120..8166
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"