LOCUS       Exported                7872 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBCGE2, complete sequence.
ACCESSION   EF175997
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Govan VA, Williamson AL.
  TITLE     Rabbits immunised with recombinant BCG expressing the cottontail 
            rabbit papillomavirus (CRPV) L2E7E2 genes induces regression of 
            established papillomas
  JOURNAL   Virus Res. 127 (1), 43-48 (2007)
  PUBMED    17451831
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Govan VA, Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2006) Institute of Infectious Disease and 
            Molecular Medicine, University of Cape Town, Anzio Road, 
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 7872)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Expression vector pBCGE2"
                     /mol_type="other DNA"
                     /label=derived from Cottontail rabbit papillomavirus E2
                     /note="derived from Cottontail rabbit papillomavirus E2"
                     /db_xref="taxon:419254"
     CDS             1877..2692
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6556..7767
                     /codon_start=1
                     /gene="E2"
                     /product="E2"
                     /label=E2
                     /note="early protein E2"
                     /protein_id="ABM68361.1"
                     /translation="MEALSQRLDSIQEELLSLYEKESTSLESQLQHWNLLRKEQVLLHF
                     CKKHGIRQLGYTPVPSLLTSQECAKQAIEMVLYIESLLRSPYSDEPWTLQDTSRERFES
                     PPQKTFKKNPAIVEVYYDGDRGNNNEYTLWGIFIIGNADGEWVKTESGVDYRGIYYVDS
                     EGNYVYYVDFSTDAGRFAANGHYDVVFQNMRLSSSVTSSPQPLVSAPEDTVPEEAPDSA
                     VPAAQKKTGPKTTRTLGRRRSRSPGVQRRPAKQRKQAAPDEADSAAGDIRPPAPEDVGR
                     RTTTVGRTPPGRNRRLRELITEASDPPVICLKGGHNQLKCLRYRLKSKHSSLFDCISTT
                     WSWVDTTSTCRLGSGRMLIKFADSEQRDKFLSRVPLPSTTQVFLGNFYGLLLLLLLIDV
                     DVVN"
     gene            6556..7767
                     /gene="E2"
                     /label=E2
     terminator      7790..7836
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"