LOCUS       Exported                4811 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBBR1MCS-Erm, complete sequence.
ACCESSION   KY368389
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Yero D, Ferrer-Navarro M, Costenaro L, Planell R, Conchillo-Sole O, 
            Sole M, Roca I, Gibert I, Daura X.
  TITLE     A transport system associated with the intrinsic antimicrobial 
            multiresistance in Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Yero D, Gibert I.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Genetica Molecular Bacteriana, Institut de 
            Biotecnologia i de Biomedicina (IBB), Campus UAB, Bellaterra, 
            Barcelona 08191, Spain
REFERENCE   3  (bases 1 to 4811)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Cloning vector pBBR1MCS-Erm"
                     /mol_type="other DNA"
                     /db_xref="taxon:2056119"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        126..144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    153..260
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     177..193
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(227..243)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             402..1139
                     /codon_start=1
                     /gene="ermB"
                     /product="erythromycin resistance protein"
                     /label=ermB
                     /note="rRNA adenine N-6-methyltransferase"
                     /protein_id="ATW75320.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            402..1139
                     /gene="ermB"
                     /label=ermB
                     /note="rRNA adenine N-6-methyltransferase"
     CDS             complement(1556..2548)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label=mob
                     /protein_id="ATW75321.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHL"
     gene            complement(1556..2548)
                     /gene="mob"
                     /label=mob
     rep_origin      2772..3541
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3542..4204
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="replication protein; involved in replication of 
                     plasmid"
                     /protein_id="ATW75322.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            3542..4204
                     /gene="rep"
                     /label=rep