LOCUS       Exported                6640 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBBR1-GFP, complete sequence.
ACCESSION   AF110459
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ouahrani-Bettache S, Porte F, Teyssier J, Liautard JP, Kohler S.
  TITLE     pBBR1-GFP: a broad-host-range vector for prokaryotic promoter 
            studies
  JOURNAL   BioTechniques 26 (4), 620-622 (1999)
  PUBMED    10343896
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Ouahrani-Bettache S, Porte F, Teyssier J, Liautard JP, Koehler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-1998) INSERM U-431, University of Montpellier II, 
            Pl. E. Bataillon, Montpellier 34095, France
REFERENCE   3  (bases 1 to 6640)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Cloning vector pBBR1-GFP"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:92689"
     misc_feature    1..2642
                     /label=from pBBR1MCS: GenBank Accession Number U02374
                     /note="from pBBR1MCS: GenBank Accession Number U02374"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2614..2630
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2686..4182
                     /label=from pSV2-Cat: GenBank Accession Number M77788
                     /note="from pSV2-Cat: GenBank Accession Number M77788"
     CDS             complement(3110..3130)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3260..3325
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(3491..4150)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol-acetyl-transferase (CAT)"
                     /label=cat
                     /protein_id="AAD28188.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(3491..4150)
                     /gene="cat"
                     /label=cat
     misc_feature    complement(4183..4204)
                     /label=from pBBR1MCS: GenBank Accession Number U02374
                     /note="from pBBR1MCS: GenBank Accession Number U02374"
     CDS             complement(4213..4929)
                     /codon_start=1
                     /gene="gfp"
                     /product="green fluorescent protein"
                     /label=gfp
                     /note="GFP; modified form mut3; from Aequorea victoria GFP:
                     GenBank Accession Number M62653"
                     /protein_id="AAD28189.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            complement(4213..4929)
                     /gene="gfp"
                     /label=gfp
                     /note="modified form mut3"
     misc_feature    4983..4997
                     /label=from pBBR1MCS: GenBank Accession number U02374
                     /note="from pBBR1MCS: GenBank Accession number U02374"
     misc_feature    4998..6446
                     /label=from pUC18: GenBank Accession Number L08752
                     /note="from pUC18: GenBank Accession Number L08752"
     CDS             complement(5569..6429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAD28190.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5569..6429)
                     /gene="bla"
                     /label=bla
     misc_feature    6447..6640
                     /label=from pBBR1MCS: GenBank Accession Number U02374
                     /note="from pBBR1MCS: GenBank Accession Number U02374"