LOCUS       Exported                6702 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBBR-IBA3plus, complete sequence.
ACCESSION   DQ266355
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     MipZ, a Spatial Regulator Coordinating Chromosome Segregation with 
            Cell Division in Caulobacter
  JOURNAL   Cell 126 (1), 147-162 (2006)
  PUBMED    16839883
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2005) Developmental Biology, Stanford University, 
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 6702)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Cloning vector pBBR-IBA3plus"
                     /mol_type="other DNA"
                     /note="similar to pASK-IBA3plus and GenBank Accession 
                     Number U25061"
                     /db_xref="taxon:356551"
     CDS             complement(261..794)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamicin-3-acetyltransferase"
                     /label=aacC1
                     /protein_id="ABB71435.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(261..794)
                     /gene="aacC1"
                     /label=aacC1
     promoter        complement(983..1011)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1201..1256
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1207..1225
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    1217..1225
                     /label=TetO1 binding site
                     /bound_moiety="TetO1"
     protein_bind    1237..1255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1247..1255
                     /label=TetO2 binding site
                     /bound_moiety="TetO2"
     CDS             1264..1287
                     /codon_start=1
                     /gene="tetA"
                     /product="TetA"
                     /label=tetA
                     /note="TetA-derived mini ORF"
                     /protein_id="ABB71439.1"
                     /translation="MNSSTKI"
     gene            1264..1287
                     /gene="tetA"
                     /label=tetA
     RBS             1295..1317
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1343..1442
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             1409..1432
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     rep_origin      1523..1978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2005..2109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2110..2970
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABB71437.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2110..2970
                     /gene="bla"
                     /label=bla
     CDS             2977..3603
                     /codon_start=1
                     /gene="tetR"
                     /product="TetR"
                     /label=tetR
                     /note="tet repressor from Tn10"
                     /protein_id="ABB71438.1"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2977..3603
                     /gene="tetR"
                     /label=tetR
     CDS             complement(4248..4910)
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /protein_id="ABB71434.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            complement(4248..4910)
                     /gene="rep"
                     /label=rep
     rep_origin      4911..5680
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             5904..206
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label=mob
                     /protein_id="ABB71436.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLAAL"
     gene            5904..206
                     /gene="mob"
                     /label=mob