LOCUS       Exported                3778 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBB-CmR-ccdB, complete sequence.
ACCESSION   HQ416902
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Fulwiler AL, Soysa DR, Ullman B, Yates PA.
  TITLE     A rapid, efficient and economical method for generating leishmanial 
            gene targeting constructs
  JOURNAL   Mol. Biochem. Parasitol. 175 (2), 209-212 (2011)
  PUBMED    21055426
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Yates PA, Fulwiler AL, Soysa DR, Ullman B.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Biochemistry & Molecular Biology, Oregon 
            Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, 
            OR 97239, USA
REFERENCE   3  (bases 1 to 3778)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Cloning vector pBB-CmR-ccdB"
                     /mol_type="other DNA"
                     /db_xref="taxon:927610"
     misc_feature    1..13
                     /label=SfiI restriction site
                     /note="SfiI restriction site"
     promoter        195..299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             300..1160
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance protein"
                     /protein_id="ADQ43427.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            300..1160
                     /gene="bla"
                     /label=bla
     rep_origin      complement(1308..1961)
                     /direction=LEFT
     rep_origin      1331..1919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2189..2201
                     /label=SfiI restriction site
                     /note="SfiI restriction site"
     CDS             complement(2208..2513)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB toxin"
                     /label=ccdB
                     /protein_id="ADQ43429.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(2208..2513)
                     /gene="ccdB"
                     /label=ccdB
     CDS             complement(2855..3514)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="chloramphenicol resistance protein"
                     /protein_id="ADQ43428.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(2855..3514)
                     /gene="cat"
                     /label=cat
     promoter        complement(3515..3617)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"