LOCUS       Exported                4494 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative vector pBaSysBioII, complete sequence.
ACCESSION   GU126429
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Botella E, Fogg M, Jules M, Piersma S, Doherty G, Hansen A, Denham 
            EL, Le Chat L, Veiga P, Bailey K, Lewis PJ, van Dijl JM, Aymerich S,
            Wilkinson AJ, Devine KM.
  TITLE     pBaSysBioII: an integrative plasmid generating gfp transcriptional 
            fusions for high-throughput analysis of gene expression in Bacillus 
            subtilis
  JOURNAL   Microbiology (Reading, Engl.) 156 (PT 6), 1600-1608 (2010)
  PUBMED    20150235
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Botella E.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2009) Smurfit Institute of Genetics, Trinity 
            College Dublin, Lincoln Place, Dublin 2, Ireland
REFERENCE   3  (bases 1 to 4494)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Integrative vector pBaSysBioII"
                     /lab_host="Bacillus subtilis"
                     /mol_type="other DNA"
                     /db_xref="taxon:687164"
     primer_bind     145..161
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    184..212
                     /label=LIC site
                     /note="LIC site"
     gene            216..1037
                     /gene="gfpmut3"
                     /label=gfpmut3
     CDS             216..932
                     /codon_start=1
                     /gene="gfpmut3"
                     /product="GFPmut3"
                     /label=gfpmut3
                     /protein_id="ACY68259.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      952..1045
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     gene            1082..2233
                     /gene="aad9"
                     /label=aad9
     CDS             1352..2119
                     /codon_start=1
                     /gene="aad9"
                     /product="spectinomycin adenyltransferase"
                     /label=aad9
                     /note="confers resistance to spectinomycin"
                     /protein_id="ACY68260.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     primer_bind     complement(2276..2292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2300..2316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2324..2354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2369..2390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2664..3282
                     /label=from pBR322
                     /note="from pBR322"
     rep_origin      complement(2678..3266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     gene            complement(3437..4367)
                     /gene="bla"
                     /label=bla
     CDS             complement(3437..4297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers resistance to ampicillin"
                     /protein_id="ACY68261.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4298..4402)
                     /gene="bla"
                     /label=AmpR promoter