LOCUS       Exported               10830 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBART-SIL6, complete sequence.
ACCESSION   JX099568
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Kooij TW, Rauch MM, Matuschewski K.
  TITLE     Expansion of experimental genetics approaches for Plasmodium berghei
            with versatile transfection vectors
  JOURNAL   Mol. Biochem. Parasitol. 185 (1), 19-26 (2012)
  PUBMED    22705315
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Kooij TWA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2012) Parasitology Unit, Max Planck Institute for 
            Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE   3  (bases 1 to 10830)
  AUTHORS   Kooij TWA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2012) Parasitology Unit, Max Planck Institute for 
            Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE   4  (bases 1 to 10830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Aug 29, 2012 this sequence version replaced JX099568.1.
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Cloning vector pBART-SIL6"
                     /mol_type="other DNA"
                     /note="used for genetic manipulation of Plasmodium berghei"
                     /db_xref="taxon:1200358"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    69..558
                     /note="SIL6L; silent intergenic locus on Plasmodium berghei
                     chromosome 6"
     misc_feature    585..638
                     /note="5MCS; multiple cloning site"
     misc_feature    672..1376
                     /gene="mCherry"
                     /note="red fluorescent protein; used for fusion protein 
                     generation"
     gene            672..1376
                     /gene="mCherry"
                     /label=mCherry
     misc_feature    1410..1439
                     /label=c-Myc epitope tag
                     /note="c-Myc epitope tag"
     CDS             1410..1439
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    1446..1475
                     /label=c-Myc epitope tag
                     /note="c-Myc epitope tag"
     CDS             1446..1475
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    1482..1511
                     /label=c-Myc epitope tag
                     /note="c-Myc epitope tag"
     CDS             1482..1511
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     3UTR           1527..1940
                     /note="3PbPPPK-DHPS; 3UTR of Plasmodium berghei 
                     hydroxymethylpterin pyrophosphokinase-dihydropteroate 
                     synthetase"
     5UTR           1962..3331
                     /note="5PbHSP70; 5UTR of Plasmodium berghei heat shock 
                     protein 70"
     misc_feature    3355..4068
                     /gene="GFP"
                     /note="green fluorescent protein; used for fusion protein 
                     generation"
     gene            3355..4068
                     /gene="GFP"
                     /label=GFP
     CDS             3358..4068
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     3UTR           4084..4613
                     /note="3PbDHFS-FPGS; 3UTR of Plasmodium berghei 
                     dihydrofolate synthase-folylpolyglutamate synthase"
     3UTR           complement(4626..5081)
                     /note="3PbDHFR-TS; 3UTR of Plasmodium berghei 
                     dihydrofolate reductase-thymidylate synthase"
     CDS             complement(5082..6803)
                     /codon_start=1
                     /product="hDHFR-yFcu fusion protein"
                     /label=hDHFR-yFcu fusion protein
                     /protein_id="AFM78648.1"
                     /translation="MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVE
                     GKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQP
                     ELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEY
                     PGVLSDVQEEKGIKYKFEVYEKNDASGGGGSGGGGSASVTGGMASKWDQKGMDIAYEEA
                     ALGYKEGGVPIGGCLINNKDGSVLGRGHNMRFQKGSATLHGEISTLENCGRLEGKVYKD
                     TTLYTTLSPCDMCTGAIIMYGIPRCVVGENVNFKSKGEKYLQTRGHEVVVVDDERCKKI
                     MKQFIDERPQDWFEDIGEASEPFKNVYLLPQTNQLLGLYTIIRNKNTTRPDFIFYSDRI
                     IRLLVEEGLNHLPVQKQIVETDTNENFEGVSFMGKICGVSIVRAGESMEQGLRDCCRSV
                     RIGKILIQRDEETALPKLFYEKLPEDISERYVFLLDPMLATGGSAIMATEVLIKRGVKP
                     ERIYFLNLICSKEGIEKYHAAFPEVRIVTGALDRGLDENKYLVPGLGDFGDRYYCV"
     mat_peptide     complement(5085..5729)
                     /product="uracil phosphoribosyltransferase"
                     /note="ScFur1; derived from Saccharomyces cerevisiae"
     mat_peptide     complement(5730..6200)
                     /product="cytosine deaminase"
                     /note="ScFcy1; derived from Saccharomyces cerevisiae"
     mat_peptide     complement(6243..6803)
                     /product="dihydrofolate reductase"
                     /note="derived from Homo sapiens"
     5UTR           complement(6804..7747)
                     /note="5PbDHFR-TS; 5UTR of Plasmodium berghei 
                     dihydrofolate reductase-thymidylate synthase"
     misc_feature    7754..8343
                     /note="5PbHSP70_duplicate; duplicated sequence of 5UTR of
                     Plasmodium berghei heat shock protein 70"
     misc_feature    8344..8406
                     /note="3MCS; multiple cloning site"
     misc_feature    8433..8906
                     /note="SIL6R; silent intergenic locus on Plasmodium berghei
                     chromosome 6"
     promoter        complement(8936..8953)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8958..8974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(9117..9745)
                     /direction=LEFT
                     /label=ColE1 plasmid replication origin
                     /note="ColE1 plasmid replication origin"
     rep_origin      complement(9135..9723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9894..10754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(9894..10556)
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /note="confers ampicillin resistance"
                     /protein_id="AFM78647.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     gene            complement(9894..10556)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(10755..10826)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(10796..10824)
                     /regulatory_class="promoter"
                     /note="Amp"