LOCUS       Exported                4977 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBARST DNA, complete sequence.
ACCESSION   AB240452
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Construction of pDESTR, a GATEWAY vector for gene disruption in 
            filamentous fungi
  JOURNAL   Curr. Microbiol. 52 (3), 210-215 (2006)
  PUBMED    16479358
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Sone T, Abe A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2005) Teruo Sone, Hokkaido University, Graduate 
            School of Agriculture; Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 
            060-8589, Japan (E-mail:sonet@chem.agr.hokudai.ac.jp, 
            Tel:81-11-706-2502, Fax:81-11-706-4961)
REFERENCE   3  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Cloning vector pBARST"
                     /mol_type="other DNA"
                     /db_xref="taxon:355281"
     rep_origin      complement(233..821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(992..1852)
                     /codon_start=1
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /protein_id="BAE93935.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(992..1852)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(1853..1957)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2063..2164
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     regulatory      2255..2640
                     /regulatory_class="promoter"
                     /gene="TrpC"
     gene            2255..2640
                     /gene="TrpC"
                     /label=TrpC
     CDS             2641..3192
                     /codon_start=1
                     /gene="bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=bar
                     /protein_id="BAE93936.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            2641..3192
                     /gene="bar"
                     /label=bar
     misc_feature    3267..4977
                     /label=GATEWAY CcdB - CmR casette for LR recombination
                     /note="GATEWAY CcdB - CmR casette for LR recombination"
     protein_bind    3270..3394
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3419..3449
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3503..4162
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4504..4809
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4850..4974)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"