LOCUS       Exported                3781 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAN, complete sequence.
ACCESSION   KF806603
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Yadong H, Chunfang X, Dongsheng Y.
  TITLE     Biobrick parts and vectors for Heterologous protein production using
            the Pichia pastoris
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Yadong H, Chunfang X, Dongsheng Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2013) Institute of Microbial Biotechnology, 
            Institute of Biomedicine, Jinan University, No. 601, Huangpu, 
            Guangzhou, Guangdong 510632, China
REFERENCE   3  (bases 1 to 3781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     Name: the biobrick plasmid backbone, pBAN vector.
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Cloning vector pBAN"
                     /mol_type="other DNA"
                     /db_xref="taxon:1453102"
     misc_feature    1..21
                     /note="Biobrick suffix; introduces EcoRI and XbaI sites"
     regulatory      22..890
                     /regulatory_class="terminator"
                     /note="derived from Aspergillus Niger glucoamylase gene; 
                     Tgla"
     misc_feature    951..1258
                     /note="5 upstream sequence Aspergillus Niger glucoamylase 
                     promoter"
     misc_feature    1259..3446
                     /label=pUC19 sequence
                     /note="pUC19 sequence"
     promoter        1290..1394
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      1325..1353
                     /regulatory_class="promoter"
                     /note="AmpR promoter"
     CDS             1395..2255
                     /codon_start=1
                     /product="lactamase"
                     /label=lactamase
                     /note="Ampicillin resistance gene"
                     /protein_id="AHH25013.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2410..3029
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      2426..3014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3302..3323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3338..3368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3376..3392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3400..3416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3447..3758
                     /note="3 upstream sequence of Aspergillus Niger 
                     glucoamylase promoter"
     misc_feature    3759..3781
                     /note="Biobrick prefix; including the SpeI and PstI sites"
     misc_feature    3760..3781
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""