LOCUS       Exported                4619 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAD24hyg, complete sequence.
ACCESSION   DQ266354
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     MipZ, a spatial regulator coordinating chromosome segregation with 
            cell division in Caulobacter
  JOURNAL   Cell 126 (1), 147-162 (2006)
  PUBMED    16839883
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2005) Developmental Biology, Stanford University, 
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2006) Developmental Biology, Stanford University, 
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   4  (bases 1 to 4619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jan 18, 2006 this sequence version replaced DQ266354.1.
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Cloning vector pBAD24hyg"
                     /mol_type="other DNA"
                     /label=similar to GenBank Accession Number X81837
                     /note="similar to GenBank Accession Number X81837"
                     /db_xref="taxon:356550"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="AraC"
                     /label=araC
                     /note="repressor"
                     /protein_id="ABB71432.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     gene            complement(96..974)
                     /gene="araC"
                     /label=araC
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     protein_bind    1003..1020
                     /label=operator O2 binding site
                     /bound_moiety="operator O2"
     regulatory      complement(1125..1153)
                     /regulatory_class="promoter"
                     /note="Pc promoter"
     protein_bind    1161..1182
                     /label=operator O1 binding site
                     /bound_moiety="operator O1"
     protein_bind    1204..1217
                     /label=CAP binding site
                     /bound_moiety="CAP"
     protein_bind    1213..1251
                     /label=operator I2+I1 binding site
                     /bound_moiety="operator I2+I1"
     regulatory      1250..1277
                     /regulatory_class="promoter"
                     /note="PBAD promoter"
     regulatory      1306..1312
                     /regulatory_class="ribosome_binding_site"
     regulatory      1367..1792
                     /regulatory_class="terminator"
                     /note="rrnB terminator"
     terminator      1569..1655
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1747..1774
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1793..1884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2058..3056
                     /codon_start=1
                     /gene="hyg"
                     /product="hygromycin phosphotransferase"
                     /label=hyg
                     /note="Hyg"
                     /protein_id="ABB71433.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPDPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     gene            2058..3056
                     /gene="hyg"
                     /label=hyg
     rep_origin      3225..4022
                     /label=derived from pBR322
                     /note="derived from pBR322"
     rep_origin      3430..4018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"