LOCUS       Exported                5414 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus transfer vector pBacPAK-His1, complete sequence, 
            beta-lactamase (bla) gene, complete cds.
ACCESSION   U55758
VERSION     .
KEYWORDS    Baculovirus transfer vector, 6xHis tag.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Kitts PA.
  TITLE     pBacPAK-His1 transfer vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an 
            order call  (415) 424-8222 or (800) 662-2566, extension 1. 
            International customers,  please contact your local distributor. For
            technical information, call  (415) 424-8222 or (800) 662-2566, 
            extension 3.
            This sequence has been compiled from information in the sequence 
            databases,  published literature and other sources, together with 
            partial sequences  obtained by CLONTECH. If you suspect there is an 
            error in this sequence,  please contact CLONTECHs Technical Service
            Department at (415) 424-8222 or  (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Baculovirus transfer vector pBacPAK-His1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:47947"
     misc_recomb     223..1050
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             223..347
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(385..990)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        1054..1145
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1155..1172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     1273..2630
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1289..2630)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      2777..3232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3514..3618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3619..4479
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicillin"
                     /label=bla
                     /protein_id="AAB02569.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3619..4479
                     /gene="bla"
                     /label=bla
     rep_origin      4650..5238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"