LOCUS       Exported                6205 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus shuttle vector pBacHTS, complete sequence.
ACCESSION   JN029539
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Choi EW, Seen DS, Song YB, Son HS, Jung NC, Huh WK, Hahn JS, Kim K, 
            Jeong JY, Lee TG.
  TITLE     AdHTS: a high-throughput system for generating recombinant 
            adenoviruses
  JOURNAL   J. Biotechnol. 162 (2-3), 246-252 (2012)
  PUBMED    23063969
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Son H.
  TITLE     BacHTS: a high-throughput system for recombinant baculovirus 
            expression
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6205)
  AUTHORS   Son H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) Seoul National University, BRNSCIENCE CO., 
            Ltd., R&D Center 3F, Golden Helix, Seoul 151-742, South Korea
REFERENCE   4  (bases 1 to 6205)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Baculovirus shuttle vector pBacHTS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1036806"
     misc_feature    1..1250
                     /label=from AcMNPV
                     /note="from AcMNPV"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(489..1094)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     regulatory      1158..1250
                     /regulatory_class="promoter"
                     /note="AcMNPV polyhedrin"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1182..1202
                     /label=baculo-F
                     /note="baculo-F"
     misc_feature    1267..1391
                     /label=attR2
                     /note="attR2"
     protein_bind    1267..1391
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1534..1839
                     /codon_start=1
                     /product="ccdB"
                     /label=ccdB
                     /protein_id="AEH04538.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    1856..1980
                     /label=attR2
                     /note="attR2"
     protein_bind    complement(1856..1980)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1994..3437
                     /label=from AcMNPV
                     /note="from AcMNPV"
     misc_recomb     2004..3361
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2020..3361)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(2026..2046)
                     /label=baculo-R
                     /note="baculo-R"
     rep_origin      3508..3963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4245..4349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4350..5210
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="bla"
                     /protein_id="AEH04537.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5381..5969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"