LOCUS       Exported                6332 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBac9-PolhEKA, complete sequence.
ACCESSION   GU827641
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Roh JY, Choi JY, Kang JN, Wang Y, Shim HJ, Liu Q, Tao X, Xu HG, Hyun
            JH, Woo SD, Jin BR, Je YH.
  TITLE     Simple purification of a foreign protein using polyhedrin fusion in 
            a baculovirus expression system
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (8), 1522-1526 (2010)
  PUBMED    20699589
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Je YH, Roh JY, Choi JY, Kang JN.
  TITLE     Simple purification of a foreign protein using polyhedrin fusion in 
            a baculovirus expression system
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6332)
  AUTHORS   Je YH, Roh JY, Choi JY, Kang JN.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2010) Agricultural Biotechnology, Seoul National 
            University, #200 Room 6117, San56-1, Sillim9-dong, Gwanak-gu, Seoul 
            151-921, Republic of Korea
REFERENCE   4  (bases 1 to 6332)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Cloning vector pBac9-PolhEKA"
                     /mol_type="other DNA"
                     /db_xref="taxon:752520"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(489..1094)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1258..1995
                     /codon_start=1
                     /product="polyhedrin"
                     /label=polyhedrin
                     /note="derived from Autographa californica 
                     nucleopolyhedrovirus"
                     /protein_id="ADF28644.1"
                     /translation="MPDYSYRPTIGRTYVYDNKYYKNLGAVIKNAKRKKHFAEHEIEEA
                     TLDPLDNYLVAEDPFLGPGKNQKLTLFKEIRNVKPDTMKLVVGWKGKEFYRETWTRFME
                     DSFPIVNDQEVMDVFLVVNMRPTRPNRCYKFLAQHALRCDPDYVPHDVIRIVEPSWVGS
                     NNEYRISLAKKGGGCPIMNLHSEYTNSFEQFIDRVIWENFYKPIVYIGTDSAEEEEILL
                     EVSLVFKVKEFAPDAPLFTGPAYE"
     CDS             2026..2040
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     misc_recomb     2131..3488
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2147..3488)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      3635..4090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      3719..4015
                     /label=M13
                     /note="M13"
     promoter        4372..4476
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4477..5337
                     /codon_start=1
                     /product="ampicillin resistant protein"
                     /label=ampicillin resistant protein
                     /protein_id="ADF28643.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5508..6096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5509..6096
                     /label=pUC
                     /note="pUC"