LOCUS       Exported                6495 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Accepter vector pBac3xP3DsRed2attP DNA, complete sequence.
ACCESSION   AB713995
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, 
            Sezutsu H, Muthulakshmi M, Nagaraju J, Kusakabe T.
  TITLE     PhiC31 integrase-mediated cassette exchange in silkworm embryos
  JOURNAL   Mol. Genet. Genomics 287 (9), 731-739 (2012)
  PUBMED    22842670
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Yonemura N, Kobayashi I, Uchino K, Sezutsu H, Tamura T, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2012) Contact:Naoyuki Yonemura National Institute 
            of Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba 
            Science City 305-8634, Japan
REFERENCE   3  (bases 1 to 6495)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Accepter vector pBac3xP3DsRed2attP"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label=synonym: Accepter plasmid pBac3xP3DsRed2attP
                     /note="synonym: Accepter plasmid pBac3xP3DsRed2attP"
                     /db_xref="taxon:1177634"
     misc_feature    705..1473
                     /label=piggyBac right arm
                     /note="piggyBac right arm"
     misc_recomb     complement(1490..1528)
                     /label=Streptomyces phage phiC31 attP sequence
                     /note="Streptomyces phage phiC31 attP sequence"
     misc_recomb     1535..1559
                     /label=lambda phage attB1 sequence
                     /note="lambda phage attB1 sequence"
     protein_bind    1535..1559
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1604..1879
                     /regulatory_class="promoter"
                     /gene="3xP3"
                     /note="Artificial Pax6 responsive promoter"
     gene            1604..1879
                     /gene="3xP3"
                     /label=3xP3
     regulatory      1874..1883
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1880..2557
                     /codon_start=1
                     /gene="DsRed2"
                     /product="red fluorescent protein"
                     /label=DsRed2
                     /protein_id="BAM36529.1"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     gene            1880..2557
                     /gene="DsRed2"
                     /label=DsRed2
     regulatory      2558..2799
                     /regulatory_class="terminator"
                     /note="SV40"
     polyA_signal    2678..2812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     complement(2818..2842)
                     /label=lambda phage attB2 sequence
                     /note="lambda phage attB2 sequence"
     protein_bind    complement(2818..2842)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(2849..2887)
                     /label=Streptomyces phage phiC31 attP sequence
                     /note="Streptomyces phage phiC31 attP sequence"
     misc_feature    3018..3696
                     /label=piggyBac left arm
                     /note="piggyBac left arm"
     primer_bind     complement(3881..3897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3905..3921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3929..3959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3974..3995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4224..4897
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5042..5902)
                     /codon_start=1
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="ampicilin antibiotic resistance"
                     /protein_id="BAM36530.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5042..5902)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(5903..6007)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6481..2
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"