LOCUS       Exported                4603 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid vector pAYE, complete sequence.
ACCESSION   KF384455
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Yee A, Tan F-L., Ginsburg D.
  TITLE     Functional display of platelet-binding VWF fragments on filamentous 
            bacteriophage
  JOURNAL   PLoS ONE 8 (9), E73518 (2013)
  PUBMED    24019925
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Yee A, Tan F-L., Ginsburg D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2013) Life Scieneces Institute, University of 
            Michigan, 210 Washtenaw Ave, Ann Arbor, MI 48109, USA
REFERENCE   3  (bases 1 to 4603)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Phagemid vector pAYE"
                     /mol_type="other DNA"
                     /db_xref="taxon:1432850"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AHB62417.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1216..1835
                     /label=ColE1
                     /note="ColE1"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      2144..2200
                     /regulatory_class="promoter"
                     /gene="lac"
     gene            2144..2200
                     /gene="lac"
                     /label=lac
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2269..3723
                     /codon_start=1
                     /transl_except="(pos: 2326 .. 2328,  aa: OTHER)"
                     /product="VWF A1-pIII fusion protein"
                     /label=VWF A1-pIII fusion protein
                     /note="stop codon is suppressed in Eshcerichia coli 
                     containing the glnV mutation (supE)"
                     /protein_id="AHB62418.1"
                     /translation="MRVLLFLLLSLFMLPAFSA*PAGAPGGQEPGGLVVPPTDAPVSPT
                     TLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMMERLRISQKWV
                     RVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKYTLFQIFSKID
                     RPEASRIALLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHANLKQIRLIEKQA
                     PENKAFVLSSVDELEQQRDEIVSYLCDLAPEAPPPTLPPDMAQVGGGRAAAAGAPVPYP
                     DPLEPRAAQGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEK
                     MANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAG
                     SNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGV
                     FAFLLYVATFMYVFSTFANILRNKES"
     sig_peptide     2269..3720
     misc_feature    2326..2328
                     /label=amber stop
                     /note="amber stop"
     primer_bind     complement(3733..3749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3944..4417
                     /label=M13
                     /note="M13"
     rep_origin      3962..4417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"