LOCUS       Exported               12408 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pAY201 DNA, complete sequence.
ACCESSION   AB526842
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Scholz P, Haring V, Wittmann-Liebold B, Ashman K, Bagdasarian M, 
            Scherzinger E.
  TITLE     Complete nucleotide sequence and gene organization of the 
            broad-host-range plasmid RSF1010
  JOURNAL   Gene 75 (2), 271-288 (1989)
  PUBMED    2653965
REFERENCE   2  (bases 1 to 12408)
  AUTHORS   Nishikawa M, Suzuki K, Yoshida K.
  TITLE     Structural and functional stability of IncP plasmids during stepwise
            transmission by trans-kingdom mating: promiscuous conjugation of 
            Escherichia coli and Saccharomyces cerevisiae
  JOURNAL   Jpn. J. Genet. 65 (5), 323-334 (1990)
  PUBMED    2248784
REFERENCE   3  (bases 1 to 12408)
  AUTHORS   Nishikawa M, Suzuki K, Yoshida K.
  TITLE     DNA integration into recipient yeast chromosomes by trans-kingdom 
            conjugation between Escherichia coli and Saccharomyces cerevisiae
  JOURNAL   Curr. Genet. 21 (2), 101-108 (1992)
  PUBMED    1568253
REFERENCE   4  (bases 1 to 12408)
  AUTHORS   Nishikawa M, Moriguchi K, Suzuki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2009) Contact:Kazuki Moriguchi Hiroshima 
            University, Department of Biological Science, Graduate School of 
            Science; Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima 739-8526, 
            Japan
REFERENCE   5  (bases 1 to 12408)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Shuttle vector pAY201"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:686267"
     source          1..5820
                     /organism="Plasmid RSF1010"
                     /mol_type="other DNA"
                     /db_xref="taxon:2504"
     source          5821..9761
                     /organism="Plasmid pACYC177"
                     /mol_type="other DNA"
                     /db_xref="taxon:28402"
     source          9762..9869
                     /organism="Plasmid RSF1010"
                     /mol_type="other DNA"
                     /db_xref="taxon:2504"
     source          9870..12408
                     /organism="Cloning vector YIp5"
                     /mol_type="other DNA"
                     /db_xref="taxon:686264"
     rep_origin      396..790
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     rep_origin      397..792
                     /label=RSF1010 origin of replication
                     /note="RSF1010 origin of replication"
     CDS             complement(817..1101)
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC"
                     /label=mobC
                     /protein_id="BAI47951.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(817..1101)
                     /gene="mobC"
                     /label=mobC
     oriT            1132..1219
                     /label=RSF1010 origin of transfer
                     /note="RSF1010 origin of transfer"
     CDS             1300..3429
                     /codon_start=1
                     /gene="mobA"
                     /product="MobA"
                     /label=mobA
                     /protein_id="BAI47952.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     gene            1300..3429
                     /gene="mobA"
                     /label=mobA
     CDS             2048..2461
                     /codon_start=1
                     /gene="mobB"
                     /product="MobB"
                     /label=mobB
                     /protein_id="BAI47953.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            2048..2461
                     /gene="mobB"
                     /label=mobB
     CDS             2458..3429
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /protein_id="BAI47954.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     gene            2458..3429
                     /gene="repB"
                     /label=repB
     CDS             3490..3702
                     /codon_start=1
                     /product="unknown protein E"
                     /label=unknown protein E
                     /note="unknown protein E gene"
                     /protein_id="BAI47955.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             3704..3910
                     /codon_start=1
                     /product="repressor protein F"
                     /label=repressor protein F
                     /note="repressor protein F gene"
                     /protein_id="BAI47956.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             3940..4779
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /protein_id="BAI47957.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            3940..4779
                     /gene="repA"
                     /label=repA
     CDS             4766..5617
                     /codon_start=1
                     /gene="repC"
                     /product="RepC"
                     /label=repC
                     /protein_id="BAI47958.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     gene            4766..5617
                     /gene="repC"
                     /label=repC
     misc_feature    5821..6137
                     /gene="bla"
                     /label=beta-lactamase, C-terminus region
                     /note="beta-lactamase, C-terminus region"
     gene            5821..6137
                     /gene="bla"
                     /label=bla
     rep_origin      6285..7114
                     /label=p15A origin of replication
                     /note="p15A origin of replication"
     rep_origin      6302..6847
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             7442..8257
                     /codon_start=1
                     /gene="aph"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph
                     /protein_id="BAI47959.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            7442..8257
                     /gene="aph"
                     /label=aph
     misc_feature    8544..9035
                     /gene="tnpR"
                     /label=transposon Tn3 resolvase, C-terminus region
                     /note="transposon Tn3 resolvase, C-terminus region"
     gene            8544..9035
                     /gene="tnpR"
                     /label=tnpR
     promoter        9113..9217
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9218..9761
                     /gene="bla"
                     /label=beta-lactamase, N-terminus region
                     /note="beta-lactamase, N-terminus region"
     gene            9218..9761
                     /gene="bla"
                     /label=bla
     promoter        9879..9907
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             9955..11145
                     /codon_start=1
                     /gene="tetC"
                     /product="tetracycline-resistance protein"
                     /label=tetC
                     /protein_id="BAI47960.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            9955..11145
                     /gene="tetC"
                     /label=tetC
     gene            11305..12408
                     /gene="URA3"
                     /label=URA3
     regulatory      11305..11526
                     /regulatory_class="promoter"
                     /gene="URA3"
                     /note="promoter region of URA3"
     promoter        11311..11526
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11527..12330
                     /codon_start=1
                     /gene="URA3"
                     /product="Ura3p"
                     /label=URA3
                     /protein_id="BAI47961.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     regulatory      12331..12408
                     /regulatory_class="terminator"
                     /gene="URA3"
                     /note="terminator region of URA3"