LOCUS       Exported                4780 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAVA319, complete sequence.
ACCESSION   AF078810
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   von Arnim AG, Deng XW, Stacey MG.
  TITLE     Cloning vectors for the expression of green fluorescent protein 
            fusion proteins in transgenic plants
  JOURNAL   Gene 221 (1), 35-43 (1998)
  PUBMED    9852947
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   von Arnim AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-1998) Botany, The University of Tennessee, HBB437,
            Knoxville, TN 37996-1100, USA
REFERENCE   3  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Cloning vector pAVA319"
                     /lab_host="Eschericia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:79669"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..704
                     /label=polylinker
                     /note="polylinker"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      705..1459
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus dual 35S promoter"
     promoter        1116..1460
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5UTR           1460..1601
                     /note="translational enhancer; 5UTR from tobacco etch 
                     virus"
     CDS             1603..2322
                     /codon_start=1
                     /gene="GFP"
                     /product="green fluorescent protein"
                     /label=GFP
                     /protein_id="AAC82461.1"
                     /translation="MGKGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYRSI"
     gene            1603..2322
                     /gene="GFP"
                     /label=GFP
     regulatory      2323..2533
                     /regulatory_class="terminator"
                     /note="cauliflower mosaic virus 35S terminator"
     misc_feature    2534..705
                     /note="Bluescript SK (+) in GenBank Accession Number 
                     X52325"
     misc_feature    2534..2576
                     /label=polylinker
                     /note="polylinker"
     promoter        complement(2595..2613)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2634..2650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2658..2674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2682..2712)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2727..2748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3036..3624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3795..4655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4656..4760)
                     /gene="bla"
                     /label=AmpR promoter