LOCUS       Exported                3452 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAV007, complete sequence.
ACCESSION   KF411445
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   White KP, Victorsen A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2013) Human Genetics, The University of Chicago, 
            5801 South Ellis Avenue, Chicago, IL 60637, USA
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Cloning vector pAV007"
                     /mol_type="other DNA"
                     /db_xref="taxon:1400556"
     CDS             1..1044
                     /codon_start=1
                     /product="AV007 fusion tag"
                     /label=AV007 fusion tag
                     /note="superfolder green fluorescent protein/FLAG/BLRP; 
                     codon optimized for expression in Drosophila"
                     /protein_id="AGV76762.1"
                     /translation="GGSGGSGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVRGE
                     GEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEG
                     YVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNV
                     YITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSK
                     DPNEKRDHMVLLEFVTAAGITLGMDELYKESSGSFLNCCPGCCMEPGSGNWSHPQFEKE
                     NLYFQSDYKDHDGDYKDHDIDYKDDDDKLEVLFQGPENLYFQGMASSLRQILDSQKMEW
                     RSNAGGS"
     primer_bind     1..22
                     /label=forward recombineering primer binding site
                     /note="forward recombineering primer binding site"
     primer_bind     164..187
                     /label=sequencing primer site
                     /note="sequencing primer site"
     primer_bind     475..498
                     /label=sequencing primer site
                     /note="sequencing primer site"
     primer_bind     629..652
                     /label=sequencing primer site
                     /note="sequencing primer site"
     CDS             778..795
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             817..840
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             841..861
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    862..927
                     /label=3xFLAG epitope
                     /note="3xFLAG epitope"
     CDS             862..927
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    928..951
                     /label=precision sequence
                     /note="precision sequence"
     CDS             928..951
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_feature    931..1511
                     /label=ECMV IRES
                     /note="ECMV IRES"
     misc_feature    952..972
                     /label=TEV site
                     /note="TEV site"
     CDS             952..972
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    973..1044
                     /note="BLRP; biotin ligase (birA) recognition peptide"
     misc_feature    1064..1649
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     gene            1652..2534
                     /gene="aphA"
                     /label=aphA
     regulatory      1652..1657
                     /regulatory_class="minus_35_signal"
                     /gene="aphA"
     regulatory      1672..1680
                     /regulatory_class="minus_10_signal"
                     /gene="aphA"
     CDS             1731..2534
                     /codon_start=1
                     /gene="aphA"
                     /product="kanamycin resistance protein"
                     /label=aphA
                     /protein_id="AGV76763.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2503..2534)
                     /label=reverse recombineering primer binding site
                     /note="reverse recombineering primer binding site"
     rep_origin      2748..3136
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     rep_origin      2748..3121
                     /allele="R6K ori"
     oriT            3296..3405
                     /note="incP origin of transfer"