LOCUS       Exported               11534 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAV-UCSF, complete sequence.
ACCESSION   JQ281544
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Greninger AL, Knudsen GM, Betegon M, Burlingame AL, Derisi JL.
  TITLE     The 3A Protein from Multiple Picornaviruses Utilizes the Golgi 
            Adaptor Protein ACBD3 To Recruit PI4KIIIbeta
  JOURNAL   J. Virol. 86 (7), 3605-3616 (2012)
  PUBMED    22258260
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Greninger AL, DeRisi JL, Knudsen GM, Miguel B, A B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Biochemistry, UCSF, 1700 4th Street, San 
            Francisco, CA 94110, USA
REFERENCE   3  (bases 1 to 11534)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Expression vector pAV-UCSF"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:1163660"
     source          1..8320
                     /organism="Aichi virus"
                     /mol_type="other DNA"
                     /db_xref="taxon:72149"
     5UTR           1..743
     CDS             744..8042
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="AFE02949.1"
                     /translation="MAATRVSRSVLAAVAHSAAHRTYHTVLDCYDRLYLNTNPHLSYPL
                     PKNSSFPCPFCQYDEQNEVLSPESLRGEGAEPCWKCSQDKPRRKYNTTPPEDWLYDSDV
                     QSWFYPETYYSDLQQKFFDKLALLSLPGAYQAKTPEERALAGALTQLLNFPSTPPLTLP
                     TTNLQRQGNSVTNIYGNGNNVTTDVGANGWAPTVSTGLGDGPVSASADSLPGRSGGASS
                     EKTHTVSGSSNKVGSRFSKWWEPAAARASESATDSAIEGIDAAGKAASKAITRKLDRPA
                     APSSTANPQPSLIALNPSATQSGNASILTGSTAPSLLAYPTATPVPLPNPDEPSQPGPS
                     GDRTWLLDTVTWSQEFTRGWNIAGSNGMQWTGLESLIFPVSTDTNWTSTSSPTAYPLPF
                     SFVRAYPDSSWAAMYNTHSMWNCGWRVQVTVNGSQFHAGALILYMVPEATTHAIQTARD
                     NAGFVFPYVILNLYESNTATIEVPYISPTPNTSSGLHAPWTFYLQVLSPLNPPPSLPTS
                     LSCSIYVTPVDSSFHGLRYLAPQHWKTRAVPGAGTFGSAVAGQELPLCGVRAYYPPNAY
                     IPAQVRDWLEFAHRPGLMATVPWTMADEPAERLGIFPVSPSAIAGTGAPISYVISLFSQ
                     WRGELAAHLLFTGSAQHYGRLVVCYTPAAPQPPSTMQEAMRGTYTVWDVNAASTLEFTI
                     PFISNSYWKTVDVNNPDALLSTTGYVSIWVQNPLVGPHTAPASALVQAFISAGESFNVR
                     LMQNPALTSQTLTEDLDAPQDTGNIENGAADNSPQPRTTFDYTGNPLPPDTKLENFFSF
                     YRLLPMGGSGAPSLSFPADEGTIIPLNPINWLKGADVSGIAAMLSCFTYIAADLRITLR
                     FSNPNDNPATMLVAFAPPGATIPLKPTRQMLSNFYMAEVPVSAATSTMVSFSIPYTSPL
                     SAIPTSYFGWEDWSGTNFGQLSSGSWGNLMLIPSLSVDSAIPFDFQLSCWVAFGNFKAW
                     VPRPPPPLPPLPTPAANAERTVAVIKQGAASATPDVDPDDRVYIVRAQRPTYVHWAIRK
                     VAPDGSAKQISLSRSGIQALVALEPPEGEPYLEILPSHWTLAELQLGNKWEYSATNNCT
                     HFVSSITGESLPNTGFSLALGIGALTAIAASAAVAVKALPGIRRQGLLTLSADTETNQT
                     LNKITESVNQAAQVVSQFDLSGPANSVSLAASDIREAAHKVASSLNGFTDVIADIKDSL
                     FTRVSDAVESGVATFLTWLVKLFGYLLVLFGSPTPMSISGLLVIICADLAPHAREFFTA
                     SGNVLSSLYYWIASKLGLSVTPQECERATLEPQGLKDFNDGALAMRNVEWIGETAWKWA
                     HRLLDWIRGKAKTDPQAKLADVHDEIMLHYSDSILALGSEKLPIDHITKSISRCRELVS
                     IAQEAKSGPHSSFLNQAIKNYTLAISQHRKCQTGPRPEPVVVYLYGPPGTGKSLLASLL
                     AQTLSQRLAGTPDDVYSPSSASCEYFDGYTGQTVHFIDDIGQDPEGRDWANFPNLVSSA
                     PFIVPMASLEEKGTHYTSKVIVVTSNFHEPNERAARSMGALRRRVHLRINVTSNGVPFD
                     PTNALNPIPGTQSKYFTAQTPLTLFQSNTVRLDRDSIWTPTFTNMDELVDAIVTRLDRS
                     TGVSNSLASLIRRQGNRVIDAEPREIPLEYADDLLEAMAHHRPVPCSLGLSQAIANNTP
                     IQQISETFWKYRKPIFTCTTFLAVLGFLCSVIPLARSLWKSKQDTPQEPQAAYSAISHQ
                     KPKPKSQKPVPTRHIQRQGISPAVPGISNNVVHVESGNGLNKNVMSGFYIFSRFLLVPT
                     HLREPHHTTLTVGADTYDWATLQTQEFGEITIVHTPTSRQYKDMRRFIGAHPHPTGLLV
                     SQFKAAPLYVRISDNRILDLDFPGVVVCKQAYGYRAATFEGLCGSPLVTDDPSGVKILG
                     LHVAGVAGTSGFSAPIHPILGQITQFATTQQSLIVPTAEVRPGVNVNRMSRLHPSPAYG
                     AFPVKKQPAPLKRNDKRLQEGVDLDTQLFLKHGKGDVTEPWPGLEAAADLYFSTFPTSL
                     PVLTQEQAIHGTPNMEGLDMGQAAGYPWNTLGRSRRSLFDEVEPGVFVPKPELQAEINQ
                     TLEDPDYVYSTFLKDELRPTAKVEQGLTRIVEAAPIHAIVAGRMLLGGLIDYMQGRPGE
                     HGSAVGCNPDVHWTSFFYAFSEFSQVYDLDYKCFDATLPSAVFTLVADHLTRITGDPRV
                     GRYIHSIRHSHHIYGNRMYDMIGGNPSGCVATSILNTIINNICVLSALIQHPDFSPSRF
                     HILAYGDDVIYATEPPIHPSFLREFYQKHTPLVVTPANKGQDFPPTSTIYEVTFLKRWF
                     VPDDVRPIYIHPVMDPDTYEQSVMWLRDGDFQDVVTSLCHLAFHSGPKTYAAWCMKVRE
                     QCLKSGFAPNFLPYSYLQLRWLNLLAA"
     mat_peptide     744..1253
                     /product="L"
     mat_peptide     1254..2360
                     /product="VP0"
     mat_peptide     2361..3032
                     /product="VP3"
     mat_peptide     3033..3791
                     /product="VP1"
     mat_peptide     3792..4199
                     /product="2A"
     mat_peptide     4200..4694
                     /product="2B"
     mat_peptide     4695..5699
                     /product="2C"
     mat_peptide     5700..5984
                     /product="3A"
     mat_peptide     5985..6065
                     /product="3B"
     mat_peptide     6066..6635
                     /product="3C"
     mat_peptide     6636..8039
                     /product="3D"
     3UTR           8043..8319
     polyA_site      8279
     primer_bind     complement(8333..8349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8562..9017
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9299..9403
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9404..10264
                     /label=Amp marker
                     /note="Amp marker"
     CDS             9404..10264
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10419..11038
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      10435..11023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11311..11332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11347..11377
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11385..11401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11409..11425
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11454..11472
                     /label=T7 promoter
                     /note="T7 promoter"
     promoter        11454..11472
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"