LOCUS       Exported                3171 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pattP-ampicillin-attP, complete sequence.
ACCESSION   EU100102
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E.
  TITLE     PhiC31 Mediated Cassette Exchange into a BAC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2007) LBI-CR, LBI-CR, Wahringer Str. 13a, Vienna 
            A-1090, Austria
REFERENCE   3  (bases 1 to 3171)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Cloning vector pattP-ampicillin-attP"
                     /mol_type="other DNA"
                     /db_xref="taxon:464922"
     intron          62..357
                     /note="synthetic intron"
     intron          98..327
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    393..666
                     /label=bovine growth hormone poly-A signal
                     /note="bovine growth hormone poly-A signal"
     misc_feature    672..722
                     /label=minimal attP site
                     /note="minimal attP site"
     promoter        887..991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             992..1852
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="ABU89801.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1853..1903
                     /label=minimal attP site
                     /note="minimal attP site"
     primer_bind     complement(2011..2027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2035..2051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2059..2089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2104..2125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2413..3001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"