LOCUS       Exported               10124 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pattB-QF-hsp70, complete sequence.
ACCESSION   HM068614
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis
  JOURNAL   Cell 141 (3), 536-548 (2010)
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Potter CJ, Tasic B, Luo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2010) Neuroscience, The Johns Hopkins School of 
            Medicine, 855 N. Wolfe Street, Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 10124)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Cloning vector pattB-QF-hsp70"
                     /mol_type="other DNA"
                     /db_xref="taxon:768489"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..4802
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This ia a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(4809..4842)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             4874..7324
                     /codon_start=1
                     /gene="QF"
                     /product="QF"
                     /label=QF
                     /note="Q-system QF transcription factor"
                     /protein_id="ADI49825.1"
                     /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL
                     TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT
                     YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA
                     ERLHARWATSRVNKSITRLLSGQAAQDPSEDGQSPSEDINVQDAGAKTSDFPHAPHLTF
                     SAPKSSTAETRTLPGPVRPPISANTLENNLQPDGTGIGKLPPNHWRLLDIYFSYTHSWL
                     PILEKKDMYQALYQYSEQGSLLPSANVESGVHAELWSALALASFQAAATAASSATGPAS
                     AAHGHDNAINPSPADISDTARKLIPLESGPFQVQHCRALLLLCLVSLGRDDWESAWLLV
                     GFAVRVLLVVRTQLPPDDDRPRPRMRALLVACFIVDTIVSMRHNVPAHLKPDDIADLPL
                     PEDGQDQWEPWTPCEGLGGEHTMLQMLRNPAYPLSTFNHLYGVTKLVALELLPRIRTSS
                     QNAPLEFRSRLQQVIGHNSPFSVFVLSQDTASAFVPTAYLTRTVYLWAAAFSEPLNEHY
                     SHLLIETLDQYQKRFGTYAIPPLIPSLLDSLLALKKQSHSSERHRRHLEELFPAYSSIW
                     PRGGRHSNTGLQPIRQLELPPTATATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYN
                     SDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTN
                     HPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDIN
                     TYEQ"
     gene            4874..7324
                     /gene="QF"
                     /label=QF
     regulatory      7331..7587
                     /regulatory_class="terminator"
                     /note="hsp70 terminator"
     misc_recomb     7627..7911
                     /label=attB site
                     /note="attB site"
     protein_bind    7648..7717
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(7936..7954)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7975..7991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7999..8015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8023..8053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8068..8089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8377..8965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9136..9996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9997..10101)
                     /gene="bla"
                     /label=AmpR promoter