LOCUS       Exported                5732 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pASRR, complete sequence.
ACCESSION   KM350158
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Park W-J., Han S-S., Kim H-S., Kim G-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2014) Interdisciplinary Graduate Program in 
            Molecular Medicine, Chonnam National University, 77 Yongbong-ro, 
            Buk-gu, Gwangju, Jeollanam-Do 500-757, Korea
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Cloning vector pASRR"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli
                     /note="derived from Escherichia coli"
                     /db_xref="taxon:1569388"
     regulatory      1..201
                     /regulatory_class="promoter"
                     /note="BEM promoter"
     CDS             210..962
                     /codon_start=1
                     /product="LuxR transcriptional regulator"
                     /label=LuxR transcriptional regulator
                     /protein_id="AIW52356.1"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN"
     regulatory      1025..1129
                     /regulatory_class="terminator"
     terminator      1030..1116
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      complement(1209..1412)
                     /regulatory_class="terminator"
     terminator      1210..1237
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1329..1415
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1764..1780
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1790..2497)
                     /codon_start=1
                     /product="SodB/mCherry fusion protein"
                     /label=SodB/mCherry fusion protein
                     /protein_id="AIW52357.1"
                     /translation="MSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    complement(2504..2591)
                     /label=derived from SodB
                     /note="derived from SodB"
     regulatory      complement(2598..2633)
                     /regulatory_class="promoter"
                     /note="Pl-o modified promoter"
     protein_bind    2611..2627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2679..2831
                     /regulatory_class="promoter"
                     /note="luxI promoter"
     ncRNA           2838..2907
                     /product="RyhB"
                     /label=antisense_RNA
                     /ncRNA_class="antisense_RNA"
     regulatory      2908..3033
                     /regulatory_class="terminator"
     terminator      2939..3025
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3144..3246
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3247..3906
                     /codon_start=1
                     /product="CAT"
                     /label=CAT
                     /protein_id="AIW52358.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4624..5243
     rep_origin      4640..5228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5414..5554
                     /label=bom
                     /note="basis of mobility region from pBR322"