LOCUS       Exported                5758 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pASD2, complete sequence.
ACCESSION   DQ898555
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Day WA Jr., Rasmussen SL, Carpenter BM, Peterson SN, Friedlander AM.
  TITLE     Microarray analysis of transposon insertion mutations in Bacillus 
            anthracis: global identification of genes required for sporulation 
            and germination
  JOURNAL   J. Bacteriol. 189 (8), 3296-3301 (2007)
  PUBMED    17277068
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Day WA Jr., Rasmussen SL, Carpenter BM, Peterson SN, Friedlander AM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2006) Bacteriology Division, USAMRIID, 1425 Porter
            St, Frederick, MD 21702, USA
REFERENCE   3  (bases 1 to 5758)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Shuttle vector pASD2"
                     /mol_type="other DNA"
                     /label=Bacillus anthracis-Escherichia coli shuttle vector
                     /note="Bacillus anthracis-Escherichia coli shuttle vector"
                     /db_xref="taxon:405927"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     57..73
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    69..1847
                     /label=omega Kan cassette
                     /note="omega Kan cassette"
     CDS             748..1542
                     /codon_start=1
                     /gene="aphA-3"
                     /product="AphA-3"
                     /label=aphA-3
                     /note="aminoglycoside phosphotransferase"
                     /protein_id="ABJ09701.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            748..1542
                     /gene="aphA-3"
                     /label=aphA-3
     primer_bind     complement(1879..1895)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1925..1943)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1979..3484
                     /label=pE194 temperature-sensitive replicon
                     /note="pE194 temperature-sensitive replicon"
     misc_feature    3485..4561
                     /label=ColE1 replicon
                     /note="ColE1 replicon"
     rep_origin      complement(3667..4255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4568..5350)
                     /codon_start=1
                     /gene="aad9"
                     /product="Spc"
                     /label=aad9
                     /note="spectinomycin resistance"
                     /protein_id="ABJ09702.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4568..5350)
                     /gene="aad9"
                     /label=aad9