LOCUS       Exported                6623 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pASapI, complete sequence.
ACCESSION   KF534756
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Purton S.
  TITLE     pASapI: an expression vector for the Chlamydomonas chloroplast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Purton S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2013) Structural & Molecular Biology, University 
            College London, Gower Street, London WC1E 6BT, United Kingdom
REFERENCE   3  (bases 1 to 6623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Expression vector pASapI"
                     /mol_type="other DNA"
                     /db_xref="taxon:1423649"
     primer_bind     complement(9..25)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        499..603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             604..1464
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3200..3798
                     /gene="atpA"
                     /label=promoter and 5UTR
                     /note="promoter and 5UTR"
     gene            3200..3798
                     /gene="atpA"
                     /label=atpA
     3UTR           3851..4257
                     /gene="rbcL"
     gene            3851..4257
                     /gene="rbcL"
                     /label=rbcL
     CDS             complement(4410..4676)
                     /codon_start=1
                     /gene="psbH"
                     /product="PsbH"
                     /label=psbH
                     /note="photosystem II subunit H"
                     /protein_id="AHA80957.1"
                     /translation="MATGTSKAKPSKVNSDFQEPGLVTPLGTLLRPLNSEAGKVLPGWG
                     TTVLMAVFILLFAAFLLIILEIYNSSLILDDVSMSWETLAKVS"
     gene            complement(4410..4676)
                     /gene="psbH"
                     /label=psbH