LOCUS       Exported                5406 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Inducible expression vector pART3, complete sequence.
ACCESSION   DQ191048
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Sandu C, Chiribau CB, Brandsch R.
  TITLE     Characterization of HdnoR, the transcriptional repressor of the 
            6-hydroxy-D-nicotine oxidase gene of Arthrobacter nicotinovorans 
            pAO1, and its DNA-binding activity in response to L- and D-nicotine 
            Derivatives
  JOURNAL   J. Biol. Chem. 278 (51), 51307-51315 (2003)
  PUBMED    14534317
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Sandu C, Chiribau CB, Sachelaru P, Brandsch R.
  TITLE     Plasmids for nicotine-dependent and -independent gene expression in 
            Arthrobacter nicotinovorans and other arthrobacter species
  JOURNAL   Appl. Environ. Microbiol. 71 (12), 8920-8924 (2005)
  PUBMED    16332890
REFERENCE   3  (bases 1 to 5406)
  AUTHORS   Sandu C, Brandsch R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2005) Molecular Biophysics, The Rockefeller 
            University, 1230 York Ave, Box 42, New York, NY 10021, USA
REFERENCE   4  (bases 1 to 5406)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Inducible expression vector pART3"
                     /lab_host="Arthrobacter nicotinovorans"
                     /mol_type="other DNA"
                     /db_xref="taxon:346226"
     regulatory      6..177
                     /regulatory_class="promoter"
                     /note="PhdnO promoter from Arthrobacter nicotinovorans 
                     pAO1"
     repeat_region   10..33
                     /label=operator 2 (IR2)
                     /note="operator 2 (IR2)"
     repeat_region   84..120
                     /label=operator 1 (IR1)
                     /note="operator 1 (IR1)"
     regulatory      88..93
                     /regulatory_class="minus_35_signal"
     regulatory      110..116
                     /regulatory_class="minus_10_signal"
     misc_feature    176..178
                     /label=translation start codon
                     /note="translation start codon"
     misc_feature    178..231
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    232..255
                     /label=8 histidine tag stretch
                     /note="8 histidine tag stretch"
     CDS             232..255
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    256..258
                     /label=translation stop codon
                     /note="translation stop codon"
     polyA_site      410..465
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     rep_origin      482..1162
                     /label=colE1 ori
                     /note="colE1 ori"
     rep_origin      complement(538..1126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1547..2362
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label=kanR
                     /note="kanamycin resistance enzyme"
                     /protein_id="ABA41003.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1547..2362
                     /gene="kanR"
                     /label=kanR
     CDS             2893..3477
                     /codon_start=1
                     /gene="hnoR"
                     /product="HnoR"
                     /label=hnoR
                     /note="repressor of the 6-D-hydroxy-nicotine oxidase"
                     /protein_id="ABA41004.1"
                     /translation="MRISTVDRRQQLIDAAIRVIRRDGVESASLRTIASEAKASLAAVH
                     VCFTNKDELMQAAAAELLQQLVRSIPRVVDGSEDVRAIAHRVMDLFWAQMVSDELNILA
                     QFEIGIWAKRNPHHGDLSRTVYSDYEKEISKLLVAAAKRQKQTIAARNVARALIVIMDG
                     CSLQYFADPSDPRHKDLCDNLVDAYLDRVGL"
     gene            2893..3477
                     /gene="hnoR"
                     /label=hnoR
     rep_origin      3500..5406
                     /note="pCG100 origin of replication region from 
                     Corynebacterium glutamicum"