LOCUS       Exported                3420 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARHS3 DNA, complete sequence.
ACCESSION   LC142817
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Okamoto S, Niki H.
  TITLE     pARHS vector sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2016) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory, Genetics Strains Research 
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/
REFERENCE   3  (bases 1 to 3420)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Cloning vector pARHS3"
                     /mol_type="other DNA"
                     /db_xref="taxon:1825574"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             66..88
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             346..558
                     /codon_start=1
                     /gene="parB"
                     /product="plasmid partition parB protein"
                     /label=parB
                     /protein_id="BAV00617.1"
                     /translation="MSSPHQDSLLPRFAQGEEGHETTTKFPCLVCVDRVSHTVDIHLSD
                     TKIAVRDSLQRRTQGGGGFHGLRIR"
     gene            346..558
                     /gene="parB"
                     /label=parB
     misc_feature    1061..1203
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      1374..1993
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(1389..1977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2148..3008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAV00618.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2148..3008)
                     /gene="bla"
                     /label=bla
     promoter        complement(3009..3113)
                     /gene="bla"
                     /label=AmpR promoter