LOCUS       Exported                6144 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARAn58, complete sequence.
ACCESSION   FJ868795
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Roth AH, Dersch P.
  TITLE     A novel expression system for intracellular production and 
            purification of recombinant affinity-tagged proteins in Aspergillus 
            niger
  JOURNAL   Appl. Microbiol. Biotechnol. 86 (2), 659-670 (2010)
  PUBMED    19908039
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Dersch P, Roth A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2009) Institute of Microbiology, Technische 
            Universitat Braunschweig, Spielmannstr. 7, Braunschweig 38106, 
            Germany
REFERENCE   3  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Cloning vector pARAn58"
                     /host="Aspergillus niger"
                     /mol_type="other DNA"
                     /db_xref="taxon:640639"
     regulatory      1..1194
                     /regulatory_class="promoter"
                     /note="suc1"
     regulatory      1245..2313
                     /regulatory_class="terminator"
                     /note="gla"
     primer_bind     complement(2345..2361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2831..2935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2936..3796
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="derived from pUC18"
                     /protein_id="ACP41232.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             join(5217..5373,5433..6100)
                     /codon_start=1
                     /gene="pyrG"
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=pyrG
                     /protein_id="ACP41233.1"
                     /translation="MSSKSHLPYAIRATNHPNPLTSKLFSIAEEKKTNVTVSADVTTSA
                     ELLDLADRLGPYIAVLKTHIDILTDLTPSTLSSLQSLATKHNFLIFEDRKFIDIGNTVQ
                     KQYHGGALRISEWAHIINCAILPGEGIVEALAQTTKSPDFKDANQRGLLILAEMTSKGS
                     LATGEYTARSVEYARKYKGFVMGFVSTRALSEVLPEQKEESEDFVVFTTGVNLSDKGDK
                     LGQQYQTPGSAVGRGADFIIAGRGIYKADDPVEAVQRYREEGWKAYEKRVGL"
     gene            5217..6100
                     /gene="pyrG"
                     /label=pyrG