LOCUS       Exported                5878 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cyanobacterial shuttle vector pAQJ4-MCS(C) DNA, complete sequence.
ACCESSION   AB480232
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Onizuka T, Endo S, Akiyama H, Kanai S, Hirano M, Yokota A, Tanaka S,
            Miyasaka H.
  TITLE     The rbcX gene product promotes the production and assembly of 
            ribulose-1,5-bisphosphate carboxylase/oxygenase of Synechococcus sp.
            PCC7002 in Escherichia coli
  JOURNAL   Plant Cell Physiol. 45 (10), 1390-1395 (2004)
  PUBMED    15564522
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Miyasaka H, Tanaka S, Onizuka T, Akiyama H.
  TITLE     Cyanobacterial shuttle vector pAQJ4-MCS(C)
  JOURNAL   Published Only in Database (2009)
REFERENCE   3  (bases 1 to 5878)
  AUTHORS   Miyasaka H, Tanaka S, Onizuka T, Akiyama H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2009) Contact:Takuo Onizuka Toray Research Center,
            Inc., Biological Science Laboratories; 1111 Tebiro, Kamakura, 
            Kanagawa 248-0036, Japan
REFERENCE   4  (bases 1 to 5878)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Cyanobacterial shuttle vector pAQJ4-MCS(C)"
                     /mol_type="other DNA"
                     /note="derived from Cloning vector pUC19 and Synechococcus 
                     sp. PCC 7002 plasmid pAQ1 (D13972)"
                     /db_xref="taxon:595659"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..401
                     /label=EcoRI cloning site
                     /note="EcoRI cloning site"
     misc_feature    402..407
                     /label=ClaI cloning site
                     /note="ClaI cloning site"
     misc_feature    408..413
                     /label=KpnI cloning site
                     /note="KpnI cloning site"
     misc_feature    412..417
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    416..421
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    422..427
                     /label=PstI cloning site
                     /note="PstI cloning site"
     misc_feature    428..433
                     /label=XbaI cloning site
                     /note="XbaI cloning site"
     CDS             complement(625..3456)
                     /codon_start=1
                     /gene="orf943"
                     /product="ORF943"
                     /function="essential for the replication of the plasmid in 
                     cyanobacteria"
                     /label=orf943
                     /protein_id="BAH22362.1"
                     /translation="MTPYEYLLYSDEPALRRNDGRLRDTWLKRYAFVEHGGWWCSGIDI
                     KTGKDSLWGCFKGDRPRKDREDKKPIKYEHPPRVATEIFTLKVDRGTWRKIAKRHKVEL
                     PETDQGFWEWVLAHPELPIIITEGAKKAGALLTAGYCAIGLPGIYNGYRTPKNDHGEPM
                     RQLRHLIPELDLLAKNNRAIAFCFDQDKKPKTIKAVNGAIQTTGALLEKAGAKVSVITW
                     HQDAKGVDDLIVEHGAKALHNRYKHRKPLAVWEMDNLTDITTQVDLTVDQRYLDIDPRA
                     IPKDAQIIFIKSAKGTGKTEWLGKIVKLAQDDCARVLVLTHRIQLAKELARRLDIDHIS
                     ELDSSPTGGALGMAMCIDSLHPDSQAHFNFMEWHGAHIVLDEIEQVLGHALGSSTCTQD
                     RAKILETFYNLILYALRTGGKLYCSDADLSPISYELIKYILDGCEFKPFTILNTYKPCL
                     EQQRDLFFYEGNDPRDLLTNLRQAIENGEKTLVFTAAQKTASTYSTQNLESLFREKYPD
                     KRILRIDAESVAEPGHPAYGCIDSLNAILPLYDIVLCSPAVETGVSIDIKDHFDSVWGM
                     GSGVQTVNGFCQGLERLRDNVPRHVWIPKFSPHSNRIANGGYTAKAIARDQHRYAELTH
                     KLIGEHAAECSGLEDSLKPFLWAYCRYAALANRGFGSYREAILNKLLSEGYVQKDLSEI
                     DPALAKDYRDELKAVKDHNYLQERVAISKVENPDDRQYEKLKRQRAKSETERHQERHGK
                     LSRSYGLTVTPELVEKDDDGWYSQLQLEYYLTVGKAFCSARDRAKYDQLQHEGFVFKPD
                     INRRSLSPKIHLLELLNIHQFLKPGVTFTGASLEGFKENCLRYAKPIKWILGRTITDKM
                     SPLEIAQALLGKLDRKLEYKGRFGSRDNRQRVYEAIAPNDQREKVFAHWLQRDQAKLGA
                     VSNPCINRFIQEA"
     gene            complement(625..3456)
                     /gene="orf943"
                     /label=orf943
     primer_bind     complement(3657..3673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3681..3697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3705..3735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3750..3771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4059..4647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4818..5678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5679..5783)
                     /gene="bla"
                     /label=AmpR promoter