LOCUS       Exported                6188 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pAQ-EX1 DNA, complete sequence.
ACCESSION   AB071392
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Miyasaka H, Nakano H, Akiyama H, Kanai S, Hirano M.
  TITLE     Production of PHA(poly hydroxyalkanoate) by the genetically 
            engineered marine cyanobacterium
  JOURNAL   Stud. Surf. Sci. Catal. 114, 237-242 (1998)
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Onizuka T, Miyasaka H, Akiyama H, Kanai S, Hirano M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2001) Takuo Onizuka, Toray Research Center, Inc., 
            Biological Science Department; 1111 Tebiro, Kamakura, Kanagawa 
            248-8555, Japan (E-mail:Takuo_Onizuka@trc.toray.co.jp, 
            Tel:81-467-32-9962, Fax:81-467-32-0414)
REFERENCE   3  (bases 1 to 6188)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Expression vector pAQ-EX1"
                     /mol_type="other DNA"
                     /note="derived from pUC19 and pAQ1(D13972); expression 
                     vector for cyanobacteria"
                     /db_xref="taxon:171696"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..708
                     /regulatory_class="promoter"
                     /note="promoter for Synechococcus sp. PCC 7002 Rubisco gene
                     (D13971)"
     misc_feature    717
                     /label=PstI cloning site
                     /note="PstI cloning site"
     misc_feature    723
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    727
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    731
                     /label=KpnI cloning site
                     /note="KpnI cloning site"
     CDS             complement(935..3766)
                     /codon_start=1
                     /gene="orf943"
                     /product="ORF943"
                     /function="essential for transformation of cyanobacteria"
                     /label=orf943
                     /protein_id="BAB68386.1"
                     /translation="MTPYEYLLYSDEPALRRNDGRLRDTWLKRYAFVEHGGWWCSGIDI
                     KTGKDSLWGCFKGDRPRKDREDKKPIKYEHPPRVATEIFTLKVDRGTWRKIAKRHKVEL
                     PETDQGFWEWVLAHPELPIIITEGAKKAGALLTAGYCAIGLPGIYNGYRTPKNDHGEPM
                     RQLRHLIPELDLLAKNNRAIAFCFDQDKKPKTIKAVNGAIQTTGALLEKAGAKVSVITW
                     HQDAKGVDDLIVEHGAKALHNRYKHRKPLAVWEMDNLTDITTQVDLTVDQRYLDIDPRA
                     IPKDAQIIFIKSAKGTGKTEWLGKIVKLAQDDCARVLVLTHRIQLAKELARRLDIDHIS
                     ELDSSPTGGALGMAMCIDSLHPDSQAHFNFMEWHGAHIVLDEIEQVLGHALGSSTCTQD
                     RAKILETFYNLILYALRTGGKLYCSDADLSPISYELIKYILDGCEFKPFTILNTYKPCL
                     EQQRDLFFYEGNDPRDLLTNLRQAIENGEKTLVFTAAQKTASTYSTQNLESLFREKYPD
                     KRILRIDAESVAEPGHPAYGCIDSLNAILPLYDIVLCSPAVETGVSIDIKDHFDSVWGM
                     GSGVQTVNGFCQGLERLRDNVPRHVWIPKFSPHSNRIANGGYTAKAIARDQHRYAELTH
                     KLIGEHAAECSGLEDSLKPFLWAYCRYAALANRGFGSYREAILNKLLSEGYVQKDLSEI
                     DPALAKDYRDELKAVKDHNYLQERVAISKVENPDDRQYEKLKRQRAKSETERHQERHGK
                     LSRSYGLTVTPELVEKDDDGWYSQLQLEYYLTVGKAFCSARDRAKYDQLQHEGFVFKPD
                     INRRSLSPKIHLLELLNIHQFLKPGVTFTGASLEGFKENCLRYAKPIKWILGRTITDKM
                     SPLEIAQALLGKLDRKLEYKGRFGSRDNRQRVYEAIAPNDQREKVFAHWLQRDQAKLGA
                     VSNPCINRFIQEA"
     gene            complement(935..3766)
                     /gene="orf943"
                     /label=orf943
     primer_bind     complement(3967..3983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3991..4007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4015..4045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4060..4081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4369..4957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5128..5988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5989..6093)
                     /gene="bla"
                     /label=AmpR promoter